ArticleThe journal of physical chemistry. B2014
Chromophore photoreduction in red fluorescent proteins is responsible for bleaching and phototoxicity.
Article in The journal of physical chemistry. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 68 citations in OpenAlex.
- Optical Detection of Cellular Signals at Material Interfaces.Advanced healthcare materials · 2026Review
- Dark-State-Mediated Photobleaching in mCherry-Based Red Fluorescent Proteins.The journal of physical chemistry letters · 2026Article
- Exploring Transient States of PAmKate to Enable Improved Cryogenic Single-Molecule Imaging.Journal of the American Chemical Society · 2024Article
- Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging.bioRxiv : the preprint server for biology · 2024Article
- Characterization of mApple as a Red Fluorescent Protein for Cryogenic Single-Molecule Imaging with Turn-Off and Turn-On Active Control Mechanisms.The journal of physical chemistry. B · 2023Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Advances in the Genetically Engineered KillerRed for Photodynamic Therapy Applications.International journal of molecular sciences · 2021Review
- Intracellular imaging and concurrent pH sensing of cancer-derived exosomes using surface-enhanced Raman scattering.Analytical and bioanalytical chemistry · 2021Article
- Light-induced oxidant production by fluorescent proteins.Free radical biology & medicine · 2018Review
- Engineering molecular imaging strategies for regenerative medicine.Bioengineering & translational medicine · 2018Review
- Green-Light-Activated Photoreaction via Genetic Hybridization of Far-Red Fluorescent Protein and Silk.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species.Nano convergence · 2018Review
- A Guide to Fluorescent Protein FRET Pairs.Sensors (Basel, Switzerland) · 2016Review
- Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric Imaging of Adherent Cells.Biophysical journal · 2016Article
- Article
- Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.PloS one · 2015Article
- KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light.PloS one · 2015Article
- Multiphoton photochemistry of red fluorescent proteins in solution and live cells.The journal of physical chemistry. B · 2014Article
- Advances in fluorescence labeling strategies for dynamic cellular imaging.Nature chemical biology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Red fluorescent proteins (RFPs) are indispensable tools for deep-tissue imaging, fluorescence resonance energy transfer applications, and super-resolution microscopy. Using time-resolved optical spectroscopy this study investigated photoinduced dynamics of three RFPs, KillerRed, mRFP, and DsRed. In all three RFPs, a new transient absorption intermediate was observed, which decays on a microsecond-millisecond time scale. This intermediate is characterized by red-shifted absorption at 1.68-1.72 eV (λmax = 720-740 nm). On the basis of electronic structure calculations, experimental evidence, and published literature, the chemical nature of the intermediate is assigned to an unusual open-shell dianionic chromophore (dianion-radical) formed via photoreduction. A doubly charged state that is not stable in the isolated (gas phase) chromophore is stabilized by the electrostatic field of the protein. Mechanistic implications for photobleaching, blinking, and phototoxicity are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.