ArticleIranian journal of basic medical sciences2014
Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice.
Article in Iranian journal of basic medical sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Osteocalcin improves testicular morphology but does not ameliorate testosterone synthesis signaling in azoospermic mice.Clinical and experimental reproductive medicine · 2024Article
- The effect of biological mechanisms of melatonin on the proliferation of spermatogonial stem cells: a systematic review.Anatomy & cell biology · 2024Review
- Melatonin Protects Mouse Type A Spermatogonial Stem Cells against Oxidative Stress via The Mitochondrial Thioredoxin System.Cell journal · 2023Article
- Current Progress in Stem Cell Therapy for Male Infertility.Stem cell reviews and reports · 2023Review
- Effect of melatonin on steroidogenesis-related enzymes expression and testosterone synthesis following CoClIranian journal of basic medical sciences · 2023Article
- Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- The Evaluation of Melatonin Effects on Mobilization and Engraftment in Autologous Hematopoietic Stem Cell Transplant Recipients; A Randomized, Double-blind and Placebo-controlled Trial.Iranian journal of pharmaceutical research : IJPR · 2021Article
- Melatonin on hypothyroidism and gonadal development in rats: a review.JBRA assisted reproduction · 2020Review
- Melatonin-pretreated adipose-derived mesenchymal stem cells efficeintly improved learning, memory, and cognition in an animal model of Alzheimer's disease.Metabolic brain disease · 2019Article
- Troxerutin protect sperm, seminiferous epithelium and pituitary-gonadal axis from torsion-detorsion injury: An experimental study.International journal of reproductive biomedicine · 2018Article
- Melatonin Regulates the Synthesis of Steroid Hormones on Male Reproduction: A Review.Molecules (Basel, Switzerland) · 2018Review
- Tissue Engineering to Improve Immature Testicular Tissue and Cell Transplantation Outcomes: One Step Closer to Fertility Restoration for Prepubertal Boys Exposed to Gonadotoxic Treatments.International journal of molecular sciences · 2018Review
- Article
- The effects of melatonin on colonization of neonate spermatogonial mouse stem cells in a three-dimensional soft agar culture system.Stem cell research & therapy · 2017Article
- Protective effects of melatonin and ghrelin on spermatogenesis: A narrative review of the literature.International journal of reproductive biomedicine · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
OBJECTIVE(S): Transplantation quality improvement and reduction of cellular damage are important goals that are now considered by researchers. Melatonin is secreted from the pineal gland and some organs such as testes. According to beneficial effects of melatonin (such as its antioxidant and antiapoptotic properties), researchers have proposed that the use of melatonin may improve transplantation quality. The aim of this study was to investigate the effects of melatonin on the spermatogonial stem cells transplantation in the azoospermic mice. MATERIALS AND
methodsThe testes of the BALB/c mice pups (6-day-old) after vitrified-thawed, were digested with enzymes (collagenase, DNaseΙ, trypsin-EDTA) to disperse the cells. The SSCs, type A, were isolated from the rest of testicular cells by MACS. Spermatogonial stem cells were labeled with PKH26 fluorescent kit. Labeled spermatogonial stem cells were transplanted into the testes of infertile mice (busulfan 40 mg/kg). The mice died two months after transplantation and the efficiency of spermatogenesis was investigated. TNP2 and hematoxyline-eosin staining were used to detect the efficiency of cell transplantation.
resultsTNP2 were detected in the samples that received melatonin and spermatogonial stem cells transplantation, simultaneously. TNP2 was not detectable in the transplant recipient mice that received placebo for 10 weeks (control group). According to hematoxyline-eosin staining, melatonin improved structure of testes.
conclusionAdministration of melatonin (20 mg/kg) simultaneously with transplantation of spermatogonial stem cells in azoospermia mouse testis increases the efficiency of transplantation and improves structural properties of the testes tissue.
Indexed as
Identifiers
24711891PMC3976745W1580581050What OpenQuestion holds
Registered trials
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