ArticleIranian journal of pharmaceutical research : IJPR2014
Overexpression of Recombinant Human Beta Interferon (rhINF-β) in Periplasmic Space of Escherichia coli.
Article in Iranian journal of pharmaceutical research : IJPR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- From Genetic Engineering to Preclinical Safety: A Study on Recombinant Human Interferons.International journal of molecular sciences · 2025Article
- Asian elephant interferons alpha and beta and their anti-herpes viral activity.Frontiers in immunology · 2025Article
- RSM-based Model to Predict Optimum Fermentation Conditions for Soluble Expression of the Antibody Fragment Derived from 4D5MOC-B Humanized Mab in SHuffle™ T7Iranian journal of pharmaceutical research : IJPR · 2021Article
- Overexpression of the recombinant human interferon-beta (Biotechnologia · 2021Article
- Engineering Pathways in Central Carbon Metabolism Help to Increase Glycan Production and ImproveBioengineering (Basel, Switzerland) · 2019Article
- Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System.Advanced pharmaceutical bulletin · 2016Article
- Cloning and Expression of Beta Subunit Gene of Phycocyanin From Spirulina platensis in Escherichia coli.Jundishapur journal of microbiology · 2015Article
- Optimization of Fermentation Conditions for Recombinant Human Interferon Beta Production by Escherichia coli Using the Response Surface Methodology.Jundishapur journal of microbiology · 2015Article
- Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells.Iranian journal of pharmaceutical research : IJPR · 2015Article
- Isolation and Characterization of a New Thermoalkalophilic Lipase from Soil Bacteria.Iranian journal of pharmaceutical research : IJPR · 2015Article
- Construction and Periplasmic Expression of the Anti-EGFRvIII ScFv Antibody Gene in Escherichia coli.Scientia pharmaceuticaArticle
- Optimization ofAvicenna journal of medical biotechnologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks or bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon (rhINF-β) was codon optimized and overexpressed as a soluble, N-terminal pelB fusion protein and secreted into the periplasmic space of Escherichia coli BL21 (DE3). The sugar, Isopropyl-β-D-thiogalactopyranoside (IPTG) was used as a chemical inducer for rhINF-β production in the shake flasks and bench top bioreactor. Timing of beta interferon expression was controlled by using the T7 promoter. The rhINF-β protein was extracted from periplasmic space by osmotic shock treatment and the expression of the beta interferon encoding gene in random selected transformants, was confirmed by western and dot blot methods. The maximum of product formation achieved at the OD600nm = 3.42 was found to be 35 % of the total protein content of the strain which translates to 0.32 g L-1. The constructed vector could efficiently overexpress the rhINF-β into the periplasmic space of E. coli. The obtained yield of the produced rhINF-β was more than previous reports. The system is easily adapted to include other vectors, tags or fusions and therefore has the potential to be broadly applicable to express other recombinant proteins.
Indexed as
Identifiers
24711841PMC3977065W1957267392What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.