ArticleIranian journal of pharmaceutical research : IJPR2014
Challenges to design and develop of DNA aptamers for protein targets. I. Optimization of asymmetric PCR for generation of a single stranded DNA library.
Article in Iranian journal of pharmaceutical research : IJPR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Progress in Aptamer Research and Future Applications.ChemistryOpen · 2025Review
- Specific Aspects of SELEX Protocol: Different Approaches for ssDNA Generation.Methods and protocols · 2025Article
- Optimized Lambda Exonuclease Digestion or Purification Using Streptavidin-Coated Beads: Which One Is Best for Successful DNA Aptamer Selection?Methods and protocols · 2022Article
- Generation and Selection of Specific Aptamers TargetingInternational journal of molecular sciences · 2022Article
- Development of an optimization pipeline of asymmetric PCR towards the generation of DNA aptamers: a guide for beginners.World journal of microbiology & biotechnology · 2022Article
- Strategies for the preparation of non-amplified and amplified genomic dengue gene samples for electrochemical DNA biosensing applications.RSC advances · 2021Article
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- Therapeutic applications of nucleic acid aptamers in microbial infections.Journal of biomedical science · 2020Review
- G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host Cells.Molecular therapy. Nucleic acids · 2018Article
- Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures.Molecules (Basel, Switzerland) · 2017Review
- Challenges to Design and Develop of DNA Aptamers for Protein Targets. II. Development of the Aptameric Affinity Ligands Specific to Human Plasma Coagulation Factor VIII Using SEC-SELEX.Iranian journal of pharmaceutical research : IJPR · 2017Article
- Aptamers and Their Significant Role in Cancer Therapy and Diagnosis.Biomedicines · 2015Review
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Authors and funding
6 authors.
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Abstract
Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers with high affinity for protein targets and develop an efficient asymmetric PCR amplification. Thus, the influence of factors including annealing temperature, number of amplification cycles, primer ratio, Mg(2+) concentration and the presence of a PCR enhancer on the amplification of the desired product were evaluated. Results obtained by agarose gel electrophoresis showed that the annealing temperature of 64 °C, Mg(2+) concentration of 0.25 mM, reverse to forward primer ratio of 15:1, amplification cycle of 25 and the presence L-ectoin as a PCR enhancer with the concentration of 0.4 M were the optimal conditions. Our results supported that the yield of this type of ssDNA production is sufficient for combinatorial screening of aptamers.
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24711839PMC3977063What OpenQuestion holds
Registered trials
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