ArticleNucleic acids research2014
The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Modulating the aroma and taste profile of soybean using novel strains for fermentation.Current research in food science · 2025Article
- A constitutive heterochromatic region shapes genome organization and impacts gene expression in Neurospora crassa.BMC genomics · 2024Article
- A Constitutive Heterochromatic Region Shapes Genome Organization and Impacts Gene Expression inbioRxiv : the preprint server for biology · 2024Article
- H3T11 phosphorylation by CKII is required for heterochromatin formation in Neurospora.Nucleic acids research · 2024Article
- Histone H3 N-Terminal Lysine Acetylation Governs Fungal Growth, Conidiation, and Pathogenicity through Regulating Gene Expression inJournal of fungi (Basel, Switzerland) · 2024Article
- Sensing of H2O2-induced oxidative stress by the UPF factor complex is crucial for activation of catalase-3 expression in Neurospora.PLoS genetics · 2023Article
- Nuclear genome organization in fungi: from gene folding to Rabl chromosomes.FEMS microbiology reviews · 2023Article
- The Growth and Conidiation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- A role for the mitotic proteins Bub3 and BuGZ in transcriptional regulation of catalase-3 expression.PLoS genetics · 2022Article
- The genome organization of Neurospora crassa at high resolution uncovers principles of fungal chromosome topology.G3 (Bethesda, Md.) · 2022Article
- H3K56 deacetylation and H2A.Z deposition are required for aberrant heterochromatin spreading.Nucleic acids research · 2022Article
- NC2 complex is a key factor for the activation of catalase-3 transcription by regulating H2A.Z deposition.Nucleic acids research · 2020Article
- The Neurospora RNA polymerase II kinase CTK negatively regulates catalase expression in a chromatin context-dependent manner.Environmental microbiology · 2020Article
- Suppression of WHITE COLLAR-independent frequency Transcription by Histone H3 Lysine 36 Methyltransferase SET-2 Is Necessary for Clock Function in Neurospora.The Journal of biological chemistry · 2016Article
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10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Neurospora crassa, the methionine synthase gene met-8 plays a key role in methionine synthesis. In this study, we found that MET-8 protein levels were compromised in several mutants defective in proper heterochromatin formation. Bioinformatics analysis revealed a 50-kb AT-rich region adjacent to the met-8 promoter. ChIP assays confirmed that trimethylated H3K9 was enriched in this region, indicating that heterochromatin may form upstream of met-8. In an H3K9R mutant strain, the output of met-8 was dramatically reduced, similar to what we observed in mutant strains that had defective heterochromatin formation. Furthermore, the production of ectopically expressed met-8 at the his-3 locus in the absence of a normal heterochromatin environment was inefficient, whereas ectopic expression of met-8 downstream of two other heterochromatin domains was efficient. In addition, our data show that the expression of mig-6 was also controlled by an upstream 4.2-kb AT-rich region similar to that of the met-8 gene, and we demonstrate that the AT-rich regions adjacent to met-8 or mig-6 are required for their peak expression. Our study indicates that met-8 and mig-6 may represent a novel type of gene, whose expression relies on the proper formation of a nearby heterochromatin region.
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