ArticleJournal of Zhejiang University. Science. B2014
Optimization of the quenching method for metabolomics analysis of Lactobacillus bulgaricus.
Article in Journal of Zhejiang University. Science. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Bacterial Metabolomics: Sample Preparation Methods.Biochemistry research international · 2022Review
- Improved Sample Preparation for Untargeted Metabolomics Profiling of Escherichia coli.Microbiology spectrum · 2021Article
- The limitless applications of single-cell metabolomics.Current opinion in biotechnology · 2021Review
- Effects of Different Sample Pulverisation Methods on the Extraction of Metabolites from the Fermented Cottonseed Meal Based on UPLC-Q-TOF-MS.Current microbiology · 2020Article
- A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation.BMC microbiology · 2018Article
- Optimization of Metabolite Extraction Protocols for the Identification and Profiling of Small Molecule Metabolites from Planktonic and BiofilmCurrent Metabolomics · 2016Article
- Metabolic fingerprinting of Lactobacillus paracasei: the optimal quenching strategy.Microbial cell factories · 2015Article
- Effect of culturing conditions on the expression of key enzymes in the proteolytic system of Lactobacillus bulgaricus.Journal of Zhejiang University. Science. B · 2015Article
- Evaluation of sample extracting methods of FCSM by Lactobacillus acidophilus based on a UPLC-Q-TOF-MS global metabolomics analysis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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Abstract
This study proposed a quenching protocol for metabolite analysis of Lactobacillus delbrueckii subsp. bulgaricus. Microbial cells were quenched with 60% methanol/water, 80% methanol/glycerol, or 80% methanol/water. The effect of the quenching process was assessed by the optical density (OD)-based method, flow cytometry, and gas chromatography-mass spectrometry (GC-MS). The principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were employed for metabolite identification. The results indicated that quenching with 80% methanol/water solution led to less damage to the L. bulgaricus cells, characterized by the lower relative fraction of prodium iodide (PI)-labeled cells and the higher OD recovery ratio. Through GC-MS analysis, higher levels of intracellular metabolites (including focal glutamic acid, aspartic acid, alanine, and AMP) and a lower leakage rate were detected in the sample quenched with 80% methanol/water compared with the others. In conclusion, we suggested a higher concentration of cold methanol quenching for L. bulgaricus metabolomics due to its decreasing metabolite leakage.
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