ArticleMolecular and cellular biology1989
Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 121 citations in OpenAlex.
- Loss of keratin 14 expression from immortalized keratinocytes by promoter methylation.Experimental dermatology · 2024Article
- Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.Translational vision science & technology · 2022Article
- Revisiting the significance of keratin expression in complex epithelia.Journal of cell science · 2022Article
- Absence of keratins 8 and 18 in rodent epithelial cell lines associates with keratin gene mutation and DNA methylation: Cell line selective effects on cell invasion.Experimental cell research · 2015Article
- Directed expression of a chimeric type II keratin partially rescues keratin 5-null mice.The Journal of biological chemistry · 2014Article
- Two mechanisms regulate keratin K15 expression in keratinocytes: role of PKC/AP-1 and FOXM1 mediated signalling.PloS one · 2012Article
- Redox regulation of cytokeratin 18 protein by NADPH oxidase 1 in preneoplastic human epithelial cells.Journal of cancer research and clinical oncology · 2011Article
- A cell culture system for the induction of Mallory bodies: Mallory bodies and aggresomes represent different types of inclusion bodies.Histochemistry and cell biology · 2009Article
- Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia.Journal of anatomy · 2009Review
- Keratin mutation predisposes to mouse liver fibrosis and unmasks differential effects of the carbon tetrachloride and thioacetamide models.Gastroenterology · 2008Article
- Keratin overexpression levels correlate with the extent of spontaneous pancreatic injury.The American journal of pathology · 2008Article
- Intermediate filaments: a historical perspective.Experimental cell research · 2007Review
- Keratin 8 overexpression promotes mouse Mallory body formation.The Journal of cell biology · 2005Article
- A small surface hydrophobic stripe in the coiled-coil domain of type I keratins mediates tetramer stability.The Journal of cell biology · 2005Article
- Intermediate cells in human prostate epithelium are enriched in proliferative inflammatory atrophy.The American journal of pathology · 2003Article
- Formation of a normal epidermis supported by increased stability of keratins 5 and 14 in keratin 10 null mice.Molecular biology of the cell · 2001Article
- Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex.Molecular biology of the cell · 2001Article
- Cytokeratins 8 and 19 in the mouse placental development.The Journal of cell biology · 2000Article
- Discovering functional relationships between RNA expression and chemotherapeutic susceptibility using relevance networks.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Human keratin 18 (K18) and keratin 8 (K8) and their mouse homologs, Endo B and Endo A, respectively, are expressed in adult mice primarily in a variety of simple epithelial cell types in which they are normally found in equal amounts within the intermediate filament cytoskeleton. Expression of K18 alone in mouse L cells or NIH 3T3 fibroblasts from either the gene or a cDNA expression vector results in K18 protein which is degraded relatively rapidly without the formation of filaments. A K8 cDNA containing all coding sequences was isolated and expressed in mouse fibroblasts either singly or in combination with K18. Immunoprecipitation of stably transfected L cells revealed that when K8 was expressed alone, it was degraded in a fashion similar to that seen previously for K18. However, expression of K8 in fibroblasts that also expressed K18 resulted in stabilization of both K18 and K8. Immunofluorescent staining revealed typical keratin filament organization in such cells. Thus, expression of a type I and a type II keratin was found to be both necessary and sufficient for formation of keratin filaments within fibroblasts. To determine whether a similar proteolytic system responsible for the degradation of K18 in fibroblasts also exists in simple epithelial cells which normally express a type I and a type II keratin, a mutant, truncated K18 protein missing the carboxy-terminal tail domain and a conserved region of the central, alpha-helical rod domain was expressed in mouse parietal endodermal cells. This resulted in destabilization of endogenous Endo A and Endo B and inhibition of the formation of typical keratin filament structures. Therefore, cells that normally express keratins contain a proteolytic system similar to that found in experimentally manipulated fibroblasts which degrades keratin proteins not found in their normal polymerized state.
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