ArticleThe American journal of tropical medicine and hygiene2014
Impact of short-time urine freezing on the sensitivity of an established Schistosoma real-time PCR assay.
Article in The American journal of tropical medicine and hygiene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic accuracy of nucleic acid amplification tests in urine for pulmonary tuberculosis: a meta-analysis.The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2015Pooled it
- Evaluation of six different tests for Schistosoma haematobium diagnosis in a near-elimination setting: A prospective observational diagnostic accuracy study.PLoS neglected tropical diseases · 2026Observational
- Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon.Diagnostics (Basel, Switzerland) · 2025Article
- Impact of -20 C° cryopreservation on serum factors from schistosomiasis patients at different storage durations: insights into serum bio-banking.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Evaluation of the Allplex GI Parasite and Helminth PCR Assay in a Belgian Travel Clinic.Diagnostics (Basel, Switzerland) · 2024Article
- Validation of artificial intelligence-based digital microscopy for automated detection of Schistosoma haematobium eggs in urine in Gabon.PLoS neglected tropical diseases · 2024Article
- Article
- Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity ofMicroorganisms · 2022Article
- Multicentric Evaluation of SeeGene Allplex Real-Time PCR Assays Targeting 28 Bacterial, Microsporidal and Parasitic Nucleic Acid Sequences in Human Stool Samples.Diagnostics (Basel, Switzerland) · 2022Article
- Schistosomiasis with a Focus on Africa.Tropical medicine and infectious disease · 2021Review
- New Developments in PCR-Based Diagnostics for Bacterial Pathogens Causing Gastrointestinal Infections-A Narrative Mini-Review on Challenges in the Tropics.Tropical medicine and infectious disease · 2021Review
- Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples.Pathogens (Basel, Switzerland) · 2021Article
- Case Report: Highlighting Male Genital Schistosomiasis (MGS) in Fishermen from the Southwestern Shoreline of Lake Malawi, Mangochi District.The American journal of tropical medicine and hygiene · 2019Article
- Article
- Improved detection of DNA Schistosoma haematobium from eggs extracted by bead beating in urine.Parasitology research · 2019Article
- Effect of sample pooling and transport conditions on the clinical sensitivity of a real-time polymerase chain reaction assay for Campylobacter fetus subsp. venerealis in preputial samples from bulls.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- The Rapid-Heat LAMPellet Method: A Potential Diagnostic Method for Human Urogenital Schistosomiasis.PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR.
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Registered trials
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