ArticlePloS one2014
Targeted genome editing of sweet orange using Cas9/sgRNA.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 179 papers.
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Who cites it
179 citing papers in PubMed.
- REF1 peptides significantly enhance transformation efficiency but inhibit shoot regeneration in citrusHorticulture research · 2026Article
- Cas9/sgRNA-mediated genome editing of citrus via mature tissue transformation enables both high-efficacy genome editing and early flowering.Frontiers in plant science · 2026Article
- Rye sprout as a host for the rapid transient production of a recombinant protein using a geminivirus DNA-containing expression vector.Plant biotechnology (Tokyo, Japan) · 2025Article
- CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.BMC plant biology · 2025Article
- Genome editing in maize and sorghum: A comprehensive review of CRISPR/Cas9 and emerging technologies.The plant genome · 2025Review
- Highly Efficient Homozygous CRISPR/Cas9 Gene Editing Based on Single-Cell-Originated Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus.Plant methods · 2024Article
- Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene.Transgenic research · 2024Article
- Optimization of CRISPR-Cas9 system iniScience · 2024Article
- Generation of transgene-free canker-resistantFrontiers in plant science · 2024Article
- Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome.Frontiers in plant science · 2024Article
- CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding.International journal of molecular sciences · 2023Review
- Apple CRISPR-Cas9-A Recipe for Successful Targeting ofPlants (Basel, Switzerland) · 2023Article
- CRISPR/Cas9-mediated disruption of CjACOS5 confers no-pollen formation on sugi trees (Cryptomeria japonica D. Don).Scientific reports · 2023Article
- Combination of Hairy Root and Whole-Plant Transformation Protocols to Achieve Efficient CRISPR/Cas9 Genome Editing in Soybean.Plants (Basel, Switzerland) · 2023Article
- Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants.Frontiers in plant science · 2023Review
- Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals.Frontiers in genome editing · 2023Review
- Advancing tree genomics to future proof next generation orchard production.Frontiers in plant science · 2023Review
- CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.Frontiers in plant science · 2023Review
- Targeted genome editing in polyploids: lessons fromFrontiers in plant science · 2023Review
119 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic modification, including plant breeding, has been widely used to improve crop yield and quality, as well as to increase disease resistance. Targeted genome engineering is expected to contribute significantly to future varietal improvement, and genome editing technologies using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9/single guide RNA (sgRNA) have already been successfully used to genetically modify plants. However, to date, there has been no reported use of any of the current genome editing approaches in sweet orange, an important fruit crop. In this study, we first developed a novel tool, Xcc-facilitated agroinfiltration, for enhancing transient protein expression in sweet orange leaves. We then successfully employed Xcc-facilitated agroinfiltration to deliver Cas9, along with a synthetic sgRNA targeting the CsPDS gene, into sweet orange. DNA sequencing confirmed that the CsPDS gene was mutated at the target site in treated sweet orange leaves. The mutation rate using the Cas9/sgRNA system was approximately 3.2 to 3.9%. Off-target mutagenesis was not detected for CsPDS-related DNA sequences in our study. This is the first report of targeted genome modification in citrus using the Cas9/sgRNA system-a system that holds significant promise for the study of citrus gene function and for targeted genetic modification.
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