ArticlePloS one2014
Long-term single cell analysis of S. pombe on a microfluidic microchemostat array.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Nonlinear memory in cell-division dynamics across species.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evolutionary Advantage of Cell Size Control.Physical review letters · 2025Article
- Intracellular signaling in proto-eukaryotes evolves to alleviate regulatory conflicts of endosymbiosis.PLoS computational biology · 2024Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- Characterizing non-exponential growth and bimodal cell size distributions in fission yeast: An analytical approach.PLoS computational biology · 2022Article
- Automated tracking of S. pombe spindle elongation dynamics.Journal of microscopy · 2021Article
- Emerging machine learning approaches to phenotyping cellular motility and morphodynamics.Physical biology · 2021Review
- Article
- Efficient computation of stochastic cell-size transient dynamics.BMC bioinformatics · 2019Article
- MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine.Scientific reports · 2019Article
- A critical analysis of Powell's results on the interdivision time distribution.Scientific reports · 2019Article
- Noisy Cell-Size-Correlated Expression of Cyclin B Drives Probabilistic Cell-Size Homeostasis in Fission Yeast.Current biology : CB · 2019Article
- Sizing up the cell cycle: systems and quantitative approaches in Chlamydomonas.Current opinion in plant biology · 2018Review
- Cell size control driven by the circadian clock and environment in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Size control in mammalian cells involves modulation of both growth rate and cell cycle duration.Nature communications · 2018Article
- Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions.Scientific reports · 2018Article
- Fundamental principles in bacterial physiology-history, recent progress, and the future with focus on cell size control: a review.Reports on progress in physics. Physical Society (Great Britain) · 2018Review
- A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells.Bio-protocol · 2018Article
- Archaeal cells share common size control with bacteria despite noisier growth and division.Nature microbiology · 2018Article
- Integrating impedance-based growth-rate monitoring into a microfluidic cell culture platform for live-cell microscopy.Microsystems & nanoengineering · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although Schyzosaccharomyces pombe is one of the principal model organisms for studying the cell cycle, surprisingly few methods have characterized S. pombe growth on the single cell level, and no methods exist capable of analyzing thousands of cells and tens of thousands of cell division events. We developed an automated microfluidic platform permitting S. pombe to be grown on-chip for several days under defined and changeable conditions. We developed an image processing pipeline to extract and quantitate several physiological parameters including cell length, time to division, and elongation rate without requiring synchronization of the culture. Over a period of 50 hours our platform analyzed over 100000 cell division events and reconstructed single cell lineages up to 10 generations in length. We characterized cell lengths and division times in a temperature shift experiment in which cells were initially grown at 30°C and transitioned to 25°C. Although cell length was identical at both temperatures at steady-state, we observed transient changes in cell length if the temperature shift took place during a critical phase of the cell cycle. We further show that cells born with normal length do divide over a wide range of cell lengths and that cell length appears to be controlled in the second generation, were large newly born cells have a tendency to divide more rapidly and thus at a normalized cell size. The platform is thus applicable to measure fine-details in cell cycle dynamics, should be a useful tool to decipher the molecular mechanism underlying size homeostasis, and will be generally applicable to study processes on the single cell level that require large numbers of precision measurements and single cell lineages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.