Evidence map›Paper›PMID 24710292›Full record

ArticleGenes2011

Identification of Genes Involved in the Glycosylation of Modified Viosamine of Flagellins in Pseudomonas syringae by Mass Spectrometry.

Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Chi L Nguyen, Fumiko Taguchi, Kazuhiro Chiku, Tadashi Ishii, Hiroshi Ono, Mitsuru Yoshida, Yuki Ichinose

Open access · goldAbstract read
In one paragraph

Article in Genes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
4.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Review
  3. Giant viruses of themicroLife · 2022
    Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. The rare sugarThe Journal of biological chemistry · 2017
    Article
  10. Gram-negative flagella glycosylation.International journal of molecular sciences · 2014
    Review
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Masanobu YamamotoNational Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. ymasanob@affrc.go.jp.
Mayumi Ohnishi-KameyamaNational Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. kameyama@affrc.go.jp.
Chi L NguyenThe Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan. linhchiagi@yahoo.com.
Fumiko TaguchiThe Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan. ftaguchi@cc.okayama-u.ac.jp.
Kazuhiro ChikuNational Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. kchiku@affrc.go.jp.
Tadashi IshiiNational Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. tishii@affrc.go.jp.
Hiroshi OnoNational Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. ono@affrc.go.jp.
Mitsuru YoshidaNational Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. mitsuru@affrc.go.jp.
Yuki IchinoseThe Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan. yuki@cc.okayama-u.ac.jp.
Food Research Institute · JPOkayama University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and that glycosylation was required for virulence. We further identified some glycosylation-related genes, including vioA, vioB, vioT, fgt1, and fgt2. In this study, we newly identified vioR and vioM in a so-called viosamine island as biosynthetic genes for glycosylation of mVio in Pta 6605 by the mass spectrometry (MS) of flagellin glycan in the respective mutants. Furthermore, characterization of the mVio-related genes and MS analyses of flagellin glycans in other pathovars of P. syringae revealed that mVio-related genes were essential for mVio biosynthesis in flagellin glycans, and that P. syringae pv. syringae B728a, which does not possess a viosamine island, has a different structure of glycan in its flagellin protein.

Identifiers

PMID24710292
PMCPMC3927599
OpenAlexW1970293567

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.