ArticleMolecular and cellular biology2014
Multisite phosphorylation of the Sum1 transcriptional repressor by S-phase kinases controls exit from meiotic prophase in yeast.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Meiotic Cytokinesis inJournal of fungi (Basel, Switzerland) · 2024Review
- Meiosis in budding yeast.Genetics · 2023Article
- Kar4 is required for the normal pattern of meiotic gene expression.PLoS genetics · 2023Article
- Kar4, the Yeast Homolog of METTL14, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Kar4 is Required for the Normal Pattern of Meiotic Gene Expression.bioRxiv : the preprint server for biology · 2023Article
- Phospho-Regulation of Meiotic Prophase.Frontiers in cell and developmental biology · 2021Review
- RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.Biochemistry · 2018Article
- The meiosis-specific Cdc20 family-member Ama1 promotes binding of the Ssp2 activator to the Smk1 MAP kinase.Molecular biology of the cell · 2018Article
- Repression of Middle Sporulation Genes inG3 (Bethesda, Md.) · 2017Article
- Ssp2 Binding Activates the Smk1 Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2017Article
- Ndt80 activates the meiotic ORC1 transcript isoform and SMA2 via a bi-directional middle sporulation element in Saccharomyces cerevisiae.RNA biology · 2016Article
- Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.Microbial cell (Graz, Austria) · 2016Article
- Autophosphorylation of the Smk1 MAPK is spatially and temporally regulated by Ssp2 during meiotic development in yeast.Molecular biology of the cell · 2015Article
- SUB1 Plays a Negative Role during Starvation Induced Sporulation Program in Saccharomyces cerevisiae.PloS one · 2015Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Activation of the meiotic transcription factor Ndt80 is a key regulatory transition in the life cycle of Saccharomyces cerevisiae because it triggers exit from pachytene and entry into meiosis. The NDT80 promoter is held inactive by a complex containing the DNA-binding protein Sum1 and the histone deacetylase Hst1. Meiosis-specific phosphorylation of Sum1 by the protein kinases Cdk1, Ime2, and Cdc7 is required for NDT80 expression. Here, we show that the S-phase-promoting cyclin Clb5 activates Cdk1 to phosphorylate most, and perhaps all, of the 11 minimal cyclin-dependent kinase (CDK) phospho-consensus sites (S/T-P) in Sum1. Nine of these sites can individually promote modest levels of meiosis, yet these sites function in a quasiadditive manner to promote substantial levels of meiosis. Two Cdk1 sites and an Ime2 site individually promote high levels of meiosis, likely by preparing Sum1 for phosphorylation by Cdc7. Chromatin immunoprecipitation reveals that the phosphorylation sites are required for removal of Sum1 from the NDT80 promoter. We also find that Sum1, but not its partner protein Hst1, is required to repress NDT80 transcription. Thus, while the phosphorylation of Sum1 may lead to dissociation from DNA by influencing Hst1, it is the presence of Sum1 on DNA that determines whether NDT80 will be expressed.
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