ArticlePloS one2014
Enhanced genetic tools for engineering multigene traits into green algae.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Transcriptional gene fusions via targeted integration at safe harbors for high transgene expression in Chlamydomonas reinhardtii.The New phytologist · 2025Article
- Efficient secretory production of recombinant proteins in microalgae using an exogenous signal peptide.Frontiers in microbiology · 2025Article
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- Efficient precision editing of endogenousCell reports methods · 2023Article
- Antimicrobial activity of the Flo peptide produced in Scenedesmus acutus and Nannochloropsis oculata.World journal of microbiology & biotechnology · 2023Article
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- Towards microalga-based superfoods: heterologous expression of zeolin inFrontiers in plant science · 2023Article
- Article
- Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil.Foods (Basel, Switzerland) · 2022Review
- Teaching an old 'doc' new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection.Frontiers in bioengineering and biotechnology · 2022Article
- Exploring the Impact of Terminators on Transgene Expression inLife (Basel, Switzerland) · 2021Article
- Droplet-based microfluidic screening and sorting of microalgal populations for strain engineering applications.Algal research · 2021Article
- Cloning ofBiology · 2020Article
- The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals.Molecules (Basel, Switzerland) · 2020Review
- mCherry Protein as an In Vivo Quantitative Reporter of Gene Expression in the Chloroplast of Chlamydomonas reinhardtii.Molecular biotechnology · 2020Article
- Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae.Microbial cell factories · 2020Article
- Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae.International journal of molecular sciences · 2020Article
- Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2.BioImpacts : BI · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic microalgae have the potential to impact many diverse biotechnological industries including energy, human and animal nutrition, pharmaceuticals, health and beauty, and specialty chemicals. However, major obstacles to sophisticated genetic and metabolic engineering in algae have been the lack of well-characterized transformation vectors to direct engineered gene products to specific subcellular locations, and the inability to robustly express multiple nuclear-encoded transgenes within a single cell. Here we validate a set of genetic tools that enable protein targeting to distinct subcellular locations, and present two complementary methods for multigene engineering in the eukaryotic green microalga Chlamydomonas reinhardtii. The tools described here will enable advanced metabolic and genetic engineering to promote microalgae biotechnology and product commercialization.
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