ArticleLaboratory investigation; a journal of technical methods and pathology2014
Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets.
Article in Laboratory investigation; a journal of technical methods and pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Preclinical Evaluation and Advancements in Vascularized Bone Tissue Engineering.Biomimetics (Basel, Switzerland) · 2025Review
- Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects.Stem cell research & therapy · 2023Review
- In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model.Materials (Basel, Switzerland) · 2021Article
- Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts.Journal of clinical medicine · 2019Review
- Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting.Journal of materials science. Materials in medicine · 2019Article
- Review
- Effects of androgen and progestin on the proliferation and differentiation of osteoblasts.Experimental and therapeutic medicine · 2018Article
- Prevascularization of natural nanofibrous extracellular matrix for engineering completely biological three-dimensional prevascularized tissues for diverse applications.Journal of tissue engineering and regenerative medicine · 2018Article
- The Self-Assembling Process and Applications in Tissue Engineering.Cold Spring Harbor perspectives in medicine · 2017Review
- Advances and perspectives in tooth tissue engineering.Journal of tissue engineering and regenerative medicine · 2017Review
- Article
- Dental cell sheet biomimetic tooth bud model.Biomaterials · 2016Article
- Double-layered cell transfer technology for bone regeneration.Scientific reports · 2016Article
- Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation.Scientific reports · 2016Article
- Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform.BioMed research international · 2016Article
- Spatio-Temporal Control of LbL Films for Biomedical Applications: From 2D to 3D.Advanced healthcare materials · 2015Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Addressing the problem of vascularization is of vital importance when engineering three-dimensional (3D) tissues. Endothelial cells are increasingly used in tissue-engineered constructs to obtain prevascularization and to enhance in vivo neovascularization. Rat bone marrow stromal cells were cultured in thermoresponsive dishes under osteogenic conditions with human umbilical vein endothelial cells (HUVECs) to obtain homotypic or heterotypic cell sheets (CSs). Cells were retrieved as sheets from the dishes after incubation at 20 °C. Monoculture osteogenic CSs were stacked on top of homotypic or heterotypic CSs, and subcutaneously implanted in the dorsal flap of nude mice for 7 days. The implants showed mineralized tissue formation under both conditions. Transplanted osteogenic cells were found at the new tissue site, demonstrating CS bone-inductive effect. Perfused vessels, positive for human CD31, confirmed the contribution of HUVECs for the neovascularization of coculture CS constructs. Furthermore, calcium quantification and expression of osteocalcin and osterix genes were higher for the CS constructs, with HUVECs demonstrating the more robust osteogenic potential of these constructs. This work demonstrates the potential of using endothelial cells, combined with osteogenic CSs, to increase the in vivo vascularization of CS-based 3D constructs for bone tissue engineering purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.