ArticleBiomaterials2014
The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Harnessing Lessons from Gel-Based and Advanced Biomaterial Therapeutics to Enable Direct Cellular Reprogramming.Gels (Basel, Switzerland) · 2026Review
- Cell reprogramming: methods, mechanisms and applications.Cell regeneration (London, England) · 2025Review
- Review
- Deep Learning for Cell Migration in Nonwoven Materials and Evaluating Gene Transfer Effects following AAV6-ND4 Transduction.Polymers · 2024Article
- Biphasic regulation of epigenetic state by matrix stiffness during cell reprogramming.Science advances · 2024Article
- Sensitivity of CNN image analysis to multifaceted measurements of neurite growth.BMC bioinformatics · 2023Article
- Reduction of Intracellular Tension and Cell Adhesion Promotes Open Chromatin Structure and Enhances Cell Reprogramming.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Impact on capsule formation for three different types of implant surface tomography.Scientific reports · 2022Article
- Investigating the Regulation of Neural Differentiation and Injury in PC12 Cells Using Microstructure Topographic Cues.Biosensors · 2021Article
- Bioengineering platforms for cell therapeutics derived from pluripotent and direct reprogramming.APL bioengineering · 2021Review
- Integrating Biomaterials and Genome Editing Approaches to Advance Biomedical Science.Annual review of biomedical engineering · 2021Article
- Microtopographical patterns promote different responses in fibroblasts and Schwann cells: A possible feature for neural implants.Journal of biomedical materials research. Part A · 2021Article
- The Influence of the Surface Topographical Cues of Biomaterials on Nerve Cells in Peripheral Nerve Regeneration: A Review.Stem cells international · 2021Review
- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Mapping regulators of cell fate determination: Approaches and challenges.APL bioengineering · 2020Review
- Conversion of mesenchymal stem cells into a canine hepatocyte-like cells by Foxa1 and Hnf4a.Regenerative therapy · 2020Article
- Review
- Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
- How to reprogram human fibroblasts to neurons.Cell & bioscience · 2020Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Cellular reprogramming holds tremendous potential for cell therapy and regenerative medicine. Recently, fibroblasts have been directly converted into induced neurons (iNs) by overexpression of the neuronal transcription factors Ascl1, Brn2 and Myt1L. Hypothesizing that cell-topography interactions could influence the fibroblast-to-neuron reprogramming process, we investigated the effects of various topographies on iNs produced by direct reprogramming. Final iN purity and conversion efficiency were increased on micrograting substrates. Neurite branching was increased on microposts and decreased on microgratings, with a simplified dendritic arbor characterized by the reduction of MAP2(+) neurites. Neurite outgrowth increased significantly on various topographies. DNA microarray analysis detected 20 differentially expressed genes in iNs reprogrammed on smooth versus microgratings, and quantitative PCR (qPCR) confirmed the upregulation of Vip and downregulation of Thy1 and Bmp5 on microgratings. Electrophysiology and calcium imaging verified the functionality of these iNs. This study demonstrates the potential of applying topographical cues to optimize cellular reprogramming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.