Evidence map›Paper›PMID 24709257›Full record

ArticleAntimicrobial agents and chemotherapy2014

Heterologous expression of thuricin CD immunity genes in Listeria monocytogenes.

Harsh Mathur, Paula M O'Connor, Paul D Cotter, Colin Hill, R Paul Ross

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Harsh MathurSchool of Microbiology, University College Cork, Cork, Ireland.
Paula M O'ConnorMoorepark Food Research Centre, Teagasc, Fermoy, County Cork, Ireland Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
Paul D CotterMoorepark Food Research Centre, Teagasc, Fermoy, County Cork, Ireland Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland c.hill@ucc.ie paul.cotter@teagasc.ie.
Colin HillSchool of Microbiology, University College Cork, Cork, Ireland Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland c.hill@ucc.ie paul.cotter@teagasc.ie.
R Paul RossMoorepark Food Research Centre, Teagasc, Fermoy, County Cork, Ireland Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
University College Cork · IE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacteriocins are ribosomally synthesized peptides that can have a narrow or broad spectrum of antimicrobial activity. Bacteriocin producers typically possess dedicated immunity systems that often consist of an ATP-binding cassette (ABC) transporter system and/or a dedicated immunity protein. Here we investigated the genes responsible for immunity to thuricin CD, a narrow-spectrum two-peptide sactibiotic produced by Bacillus thuringiensis DPC6431. Heterologous expression of putative thuricin CD immunity determinants allowed us to identify and investigate the relative importance of the individual genes and gene products that contribute to thuricin CD immunity. We established that TrnF and TrnG are the individual components of an ABC transporter system that provides immunity to thuricin CD. We also identified a hitherto overlooked open reading frame located upstream of trnF predicted to encode a 79-amino-acid transmembrane protein. We designated this newly discovered gene trnI and established that TrnI alone can provide protection against thuricin CD.

Indexed as

ATP-Binding Cassette TransportersBacillus thuringiensisBacterial ProteinsBacteriocinsBase SequenceGene ExpressionListeria monocytogenesMicrobial Sensitivity TestsMolecular Sequence DataOpen Reading FramesSequence Analysis, DNATransgenesATP-Binding Cassette TransportersBacterial ProteinsBacteriocinsthuricin

Identifiers

PMID24709257
PMCPMC4068476
OpenAlexW2143601415

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.