ArticleVeterinary research2014
Perfrin, a novel bacteriocin associated with netB positive Clostridium perfringens strains from broilers with necrotic enteritis.
Article in Veterinary research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Bacteriocins, a New Generation of Sustainable Alternatives to Antibacterial Agents in Primary Food Production Systems.Molecules (Basel, Switzerland) · 2026Review
- Comprehensive analysis of blood proteome response to necrotic enteritis in broiler chicken.Veterinary research · 2025Article
- Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic.Poultry science · 2024Article
- Effects of Treatment with Lactobacilli on Necrotic Enteritis in Broiler Chickens.Probiotics and antimicrobial proteins · 2022Article
- Necrotic Enteritis in Broiler Chickens: A Review on the Pathogen, Pathogenesis, and Prevention.Microorganisms · 2022Review
- A Poultry Subclinical Necrotic Enteritis Disease Model Based on NaturalFrontiers in physiology · 2022Article
- Article
- Research Note: The administration schedule of coccidia is a major determinant in broiler necrotic enteritis models.Poultry science · 2021Article
- Last Call for Replacement of Antimicrobials in Animal Production: Modern Challenges, Opportunities, and Potential Solutions.Antibiotics (Basel, Switzerland) · 2020Review
- Review
- Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged withAnimals : an open access journal from MDPI · 2020Article
- Probiotics mitigating subclinical necrotic enteritis (SNE) as potential alternatives to antibiotics in poultry.AMB Express · 2020Review
- Draft genome sequence data ofData in brief · 2018Article
- Whole genome analysis reveals the diversity and evolutionary relationships between necrotic enteritis-causing strains of Clostridium perfringens.BMC genomics · 2018Article
- Inhibition ofKorean journal for food science of animal resources · 2018Article
- Conjugation-Mediated Horizontal Gene Transfer of Clostridium perfringens Plasmids in the Chicken Gastrointestinal Tract Results in the Formation of New Virulent Strains.Applied and environmental microbiology · 2017Article
- Antimicrobial potential of bacteriocins in poultry and swine production.Veterinary research · 2017Review
- Article
- Addressing the Antibiotic Resistance Problem with Probiotics: Reducing the Risk of Its Double-Edged Sword Effect.Frontiers in microbiology · 2016Review
- Towards the control of necrotic enteritis in broiler chickens with in-feed antibiotics phasing-out worldwide.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2015Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Necrotic enteritis in broiler chickens is associated with netB positive Clostridium perfringens type A strains. It is known that C. perfringens strains isolated from outbreaks of necrotic enteritis are more capable of secreting factors inhibiting growth of other C. perfringens strains than strains isolated from the gut of healthy chickens. This characteristic could lead to extensive and selective presence of a strain that contains the genetic make-up enabling to secrete toxins that cause gut lesions. This report describes the discovery, purification, characterization and recombinant expression of a novel bacteriocin, referred to as perfrin, produced by a necrotic enteritis-associated netB-positive C. perfringens strain. Perfrin is a 11.5 kDa C-terminal fragment of a 22.9 kDa protein and showed no sequence homology to any currently known bacteriocin. The 11.5 kDa fragment can be cloned into Escherichia coli, and expression yielded an active peptide. PCR detection of the gene showed its presence in 10 netB-positive C. perfringens strains of broiler origin, and not in other C. perfringens strains tested (isolated from broilers, cattle, sheep, pigs, and humans). Perfrin and NetB are not located on the same genetic element since NetB is plasmid-encoded and perfrin is not. The bacteriocin has bactericidal activity over a wide pH-range but is thermolabile and sensitive to proteolytic digestion (trypsin, proteinase K). C. perfringens bacteriocins, such as perfrin, can be considered as an additional factor involved in the pathogenesis of necrotic enteritis in broilers.
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Registered trials
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