ArticleJournal of radiation research2014
Cranial irradiation induces bone marrow-derived microglia in adult mouse brain tissue.
Article in Journal of radiation research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Role of microglia in brain development after viral infection.Frontiers in cell and developmental biology · 2024Review
- Role of microglia in HIV-1 infection.AIDS research and therapy · 2023Review
- In Vivo Imaging of the Microglial Landscape After Whole Brain Radiation Therapy.International journal of radiation oncology, biology, physics · 2021Article
- Review
- Deletion of Neuropilin 1 from Microglia or Bone Marrow-Derived Macrophages Slows Glioma Progression.Cancer research · 2018Article
- Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid β.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Friend or Foe? Resident Microglia vs Bone Marrow-Derived Microglia and Their Roles in the Retinal Degeneration.Molecular neurobiology · 2017Review
- Integrin-Kindlin3 requirements for microglial motility in vivo are distinct from those for macrophages.JCI insight · 2017Article
- Systemic Injection of RPE65-Programmed Bone Marrow-Derived Cells Prevents Progression of Chronic Retinal Degeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Brain metastasis: Unique challenges and open opportunities.Biochimica et biophysica acta. Reviews on cancer · 2017Review
- Brain radiation injury leads to a dose- and time-dependent recruitment of peripheral myeloid cells that depends on CCR2 signaling.Journal of neuroinflammation · 2016Article
- Leukocyte telomere length and hippocampus volume: a meta-analysis.F1000Research · 2015Article
- Systemic influences contribute to prolonged microvascular rarefaction after brain irradiation: a role for endothelial progenitor cells.American journal of physiology. Heart and circulatory physiology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Postnatal hematopoietic progenitor cells do not contribute to microglial homeostasis in adult mice under normal conditions. However, previous studies using whole-body irradiation and bone marrow (BM) transplantation models have shown that adult BM cells migrate into the brain tissue and differentiate into microglia (BM-derived microglia; BMDM). Here, we investigated whether cranial irradiation alone was sufficient to induce the generation of BMDM in the adult mouse brain. Transgenic mice that express green fluorescent protein (GFP) under the control of a murine stem cell virus (MSCV) promoter (MSCV-GFP mice) were used. MSCV-GFP mice express GFP in BM cells but not in the resident microglia in the brain. Therefore, these mice allowed us to detect BM-derived cells in the brain without BM reconstitution. MSCV-GFP mice, aged 8-12 weeks, received 13.0 Gy irradiation only to the cranium, and BM-derived cells in the brain were quantified at 3 and 8 weeks after irradiation. No BM-derived cells were detected in control non-irradiated MSCV-GFP mouse brains, but numerous GFP-labeled BM-derived cells were present in the brain stem, basal ganglia and cerebral cortex of the irradiated MSCV-GFP mice. These BM-derived cells were positive for Iba1, a marker for microglia, indicating that GFP-positive BM-derived cells were microglial in nature. The population of BMDM was significantly greater at 8 weeks post-irradiation than at 3 weeks post-irradiation in all brain regions examined. Our results clearly show that cranial irradiation alone is sufficient to induce the generation of BMDM in the adult mouse.
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