ArticleProceedings of the National Academy of Sciences of the United States of America2014
Abscisic acid sensor RCAR7/PYL13, specific regulator of protein phosphatase coreceptors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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53 citing papers in PubMed, 124 citations in OpenAlex.
- Genome-wide identification and expression analysis of the PYL gene family in response to salt, drought and cold stresses in Solanum melongena L.BMC genomics · 2025Article
- Deciphering ABA/PYL gene family in flax: evolutionary analysis, and abiotic stress response.Plant cell reports · 2025Article
- Construction of a Membrane Yeast Two-Hybrid Library and Screening of MsPYR1-Like Interacting Proteins in Malus sieversii.Molecular biotechnology · 2025Article
- Genome-wide identification of pyrabactin resistance 1-like (PYL) gene family under phytohormones and drought stresses in alfalfa (Medicago sativa).BMC genomics · 2025Article
- From Regulation to Application: The Role of Abscisic Acid in Seed and Fruit Development and Agronomic Production Strategies.International journal of molecular sciences · 2024Review
- Genome-wide identification, abiotic stress, and expression analysis of PYL family in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) during grain development.BMC plant biology · 2024Article
- Article
- OsNAC120 balances plant growth and drought tolerance by integrating GA and ABA signaling in rice.Plant communications · 2024Article
- Functional characterization ofFrontiers in plant science · 2024Article
- Genome-wide identification of the PYL gene family of tea plants (Camellia sinensis) revealed its expression profiles under different stress and tissues.BMC genomics · 2023Article
- GhPYL9-5D and GhPYR1-3 A positively regulate Arabidopsis and cotton responses to ABA, drought, high salinity and osmotic stress.BMC plant biology · 2023Article
- Exogenous gibberellic acid shortening after-ripening process and promoting seed germination in a medicinal plant Panax notoginseng.BMC plant biology · 2023Article
- Article
- CRISPR/Cas9-Mediated Multiple Knockouts in Abscisic Acid Receptor Genes Reduced the Sensitivity to ABA during Soybean Seed Germination.International journal of molecular sciences · 2022Article
- Transcription factor OsNAC016: a convergent point of brassinosteroid and abscisic acid signaling in rice.Plant physiology · 2022Article
- OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8.Plant physiology · 2022Article
- Genome-wide identification and comparative analysis of the PYL gene family in eight Rosaceae species and expression analysis of seeds germination in pear.BMC genomics · 2022Article
- The Protein Phosphatase GhAP2C1 Interacts Together with GhMPK4 to Synergistically Regulate the Immune Response toInternational journal of molecular sciences · 2022Article
- Increasing yield on dry fields: molecular pathways with growing potential.The Plant journal : for cell and molecular biology · 2022Review
- Comprehensive functional analysis of the PYL-PP2C-SnRK2s family inFrontiers in plant science · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant hormone abscisic acid (ABA) acts both as a developmental signal and as an integrator of environmental cues such as drought and cold. ABA perception recruits an ABA-binding regulatory component [regulatory component of ABA receptor (RCAR)/PYR1/PYL] and an associated protein phosphatase 2C (PP2C). Phytohormone binding inactivates the phosphatase activity of the coreceptor, permitting phosphorelay of the ABA signal via downstream protein kinases. RCARs and PP2C coreceptors are represented by small protein families comprising 14 and 9 members in Arabidopsis, respectively. The specificity of the RCAR-PP2C interaction and the constraints contributing to specific combinations are poorly understood. In this contribution, we analyzed RCAR7/PYL13, which is characterized by three variant amino acid residues in the conserved ABA-binding pocket. RCAR7 regulated the phosphatase activity of the PP2Cs ABI1, ABI2, and PP2CA in vitro at nanomolar ABA levels; however, it was unable to regulate the structurally related hypersensitive to ABA 1 (HAB1). Site-directed mutagenesis of HAB1 established ABA-dependent regulation by RCAR7. Conversion of the noncanonical amino acid residues of RCAR7 into the consensus ABA-binding pocket did not perceptibly change receptor function. Ectopic expression of RCAR7 in Arabidopsis resulted in ABA hypersensitivity affecting gene regulation, seed germination, and stomatal closure. The RCAR7 loss-of-function mutant revealed no changes in ABA responses, similar to the RCAR9 knockout line, whereas the combined deficiency of RCAR7 and RCAR9 resulted in ABA-insensitive seed germination. The study shows a role of RCAR7 in early plant development, proves its ABA receptor function, and identifies structural constraints of RCAR7-PP2C interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.