ArticleProceedings of the National Academy of Sciences of the United States of America2014
Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- The Potential of Mesenchymal Stem/Stromal Cells in Diabetic Wounds and Future Directions for Research and Therapy-Is It Time for Use in Everyday Practice?International journal of molecular sciences · 2024Review
- Photo-click hydrogels for 3D in situ differentiation of pancreatic progenitors from induced pluripotent stem cells.Stem cell research & therapy · 2023Article
- Advanced cryopreservation engineering strategies: the critical step to utilize stem cell products.Cell regeneration (London, England) · 2023Review
- Progress and emerging techniques for biomaterial-based derivation of mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs).Biomaterials research · 2023Review
- Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells.Bioengineering (Basel, Switzerland) · 2022Review
- Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Engineering hiPSC-CM and hiPSC-EC laden 3D nanofibrous splenic hydrogel for improving cardiac function through revascularization and remuscularization in infarcted heart.Bioactive materials · 2021Article
- Review
- A 3-D hydrogel based system for hematopoietic differentiation and its use in modeling down syndrome associated transient myeloproliferative disorder.Biomaterials science · 2021Article
- Human-scale tissues with patterned vascular networks by additive manufacturing of sacrificial sugar-protein composites.Acta biomaterialia · 2020Article
- Present and future of tissue engineering scaffolds for dentin-pulp complex regeneration.Journal of tissue engineering and regenerative medicine · 2019Review
- Application of induced pluripotent stem cells for cartilage regeneration in CLAWN miniature pig osteochondral replacement model.Regenerative therapy · 2018Article
- Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.Molecular biotechnology · 2018Review
- Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array.Journal of visualized experiments : JoVE · 2018Article
- Why the impact of mechanical stimuli on stem cells remains a challenge.Cellular and molecular life sciences : CMLS · 2018Review
- New substrates for stem cell control.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche.Annual review of biomedical engineering · 2018Review
- The Physical and Biochemical Properties of the Extracellular Matrix Regulate Cell Fate.Current topics in developmental biology · 2018Review
- Native-mimicking in vitro microenvironment: an elusive and seductive future for tumor modeling and tissue engineering.Journal of biological engineering · 2018Review
- Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment.Chemical reviews · 2017Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the conditions required for self-renewal vs. differentiation of HPSCs are different, and a single system that efficiently achieves both outcomes is not available [Giobbe GG, et al. (2012) Biotechnol Bioeng 109(12):3119-3132]. We have addressed this dual need by developing a hydrogel-based material that uses ionic de-cross-linking to remove a self-renewal permissive hydrogel (alginate) and switch to a differentiation-permissive microenvironment (collagen). Adjusting the timing of this switch can preferentially steer the HPSC differentiation to mimic lineage commitment during gastrulation to ectoderm (early switch) or mesoderm/endoderm (late switch). As an exemplar differentiated cell type, we showed that directing early lineage specification using this single system can promote cardiogenesis with increased gene expression in high-density cell populations. This work will facilitate regenerative medicine by allowing in situ HPSC expansion to be coupled with early lineage specification within defined tissue geometries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.