ArticleProceedings of the National Academy of Sciences of the United States of America2014
A cyclic HSV1-TK reporter for real-time PET imaging of apoptosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- PET reporter systems for the brain.Trends in neurosciences · 2023Review
- Review
- Neuroinflammation in acute hepatic encephalopathy rats: imaging and therapeutic effectiveness evaluation usingMetabolic brain disease · 2018Article
- Radionuclide Imaging-Guided Chemo-Radioisotope Synergistic Therapy Using aMolecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Noninvasive bioluminescence imaging of the dynamics of sanguinarine induced apoptosis via activation of reactive oxygen species.Oncotarget · 2016Article
- Combined image guided monitoring the pharmacokinetics of rapamycin loaded human serum albumin nanoparticles with a split luciferase reporter.Nanoscale · 2016Article
- Design of a functional cyclic HSV1-TK reporter and its application to PET imaging of apoptosis.Nature protocols · 2015Article
- Molecular imaging of stem cells for the treatment of acute myocardial infarction.International journal of clinical and experimental medicine · 2015Article
- Molecular imaging of apoptosis: from micro to macro.Theranostics · 2015Review
- Bioinspired nanocomplex for spatiotemporal imaging of sequential mRNA expression in differentiating neural stem cells.ACS nano · 2014Article
- Generation and screening of monoclonal antibodies for immunoPET imaging of IGF1R in prostate cancer.Molecular pharmaceutics · 2014Article
- [(18)F]DPA-714 PET imaging of AMD3100 treatment in a mouse model of stroke.Molecular pharmaceutics · 2014Article
- Chelator-free (64)Cu-integrated gold nanomaterials for positron emission tomography imaging guided photothermal cancer therapy.ACS nano · 2014Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The coordination of cell proliferation and programmed death (apoptosis) is essential for normal physiology, and imbalance in these two opposing processes is implicated in various diseases. Objective and quantitative noninvasive imaging of apoptosis would significantly facilitate rapid screening as well as validation of therapeutic chemicals. Herein, we molecularly engineered an apoptosis switch-on PET-based cyclic herpes simplex virus type 1-thymidine kinase reporter (cTK266) containing a caspase-3 recognition domain as the switch. Translation of the reporter and protein splicing in healthy mammalian cells produce an inactive cyclic chimera. Upon apoptosis, caspase-3-specific cleavage of the circular product occurs, resulting in the restoration of the thymidine kinase activity, which can be detected in living cells and animals by noninvasive PET imaging. Our results showed the high sensitivity of this reporter in dynamic and quantitative imaging of apoptosis in living subjects. This reporter could be applied as a valuable tool for high-throughput functional screening of proapoptotic and antiapoptotic compounds in preclinical models in drug development, and monitoring the destination of therapeutic cells in clinical settings.
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