ArticleProceedings of the National Academy of Sciences of the United States of America2014
Engineering of yeast hexose transporters to transport D-xylose without inhibition by D-glucose.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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Who cites it
123 citing papers in PubMed, 292 citations in OpenAlex.
- Progresses of transposon research inSynthetic and systems biotechnology · 2026Review
- A two-step mechanism for sugar translocation.Nature structural & molecular biology · 2026Article
- Engineering mixed sugar metabolic channels inSynthetic and systems biotechnology · 2026Article
- Docking-guided and phosphomimetic rational engineering of glucose-insensitive xylose transporters from Trichoderma reesei.Microbial cell factories · 2026Article
- Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose.FEMS yeast research · 2026Article
- Engineering the acid-tolerant yeastFrontiers in microbiology · 2026Article
- Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain ofSynthetic and systems biotechnology · 2025Article
- The Saccharomyces cerevisiae amino acid transporter Lyp1 has a broad substrate spectrum.FEBS letters · 2025Article
- Glucose receptor deletion and engineering: impact on xylose sensing and utilization in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
- Advances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production.Applied microbiology and biotechnology · 2025Review
- A previously unidentified sugar transporter for engineering of high-yield Streptomyces.Applied microbiology and biotechnology · 2024Article
- Specialization Restricts the Evolutionary Paths Available to Yeast Sugar Transporters.Molecular biology and evolution · 2024Article
- Specialization restricts the evolutionary paths available to yeast sugar transporters.bioRxiv : the preprint server for biology · 2024Article
- Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium.Nature communications · 2024Article
- Nicotinic acid availability impacts redox cofactor metabolism in Saccharomyces cerevisiae during alcoholic fermentation.FEMS yeast research · 2024Article
- Maltose accumulation-induced cell death in Saccharomyces cerevisiae.FEMS yeast research · 2024Article
- Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose.FEMS yeast research · 2024Article
- Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum.Extremophiles : life under extreme conditions · 2024Article
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
63 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All known D-xylose transporters are competitively inhibited by D-glucose, which is one of the major reasons hampering simultaneous fermentation of D-glucose and D-xylose, two primary sugars present in lignocellulosic biomass. We have set up a yeast growth-based screening system for mutant D-xylose transporters that are insensitive to the presence of D-glucose. All of the identified variants had a mutation at either a conserved asparagine residue in transmembrane helix 8 or a threonine residue in transmembrane helix 5. According to a homology model of the yeast hexose transporter Gal2 deduced from the crystal structure of the D-xylose transporter XylE from Escherichia coli, both residues are found in the same region of the protein and are positioned slightly to the extracellular side of the central sugar-binding pocket. Therefore, it is likely that alterations sterically prevent D-glucose but not D-xylose from entering the pocket. In contrast, changing amino acids that are supposed to directly interact with the C6 hydroxymethyl group of D-glucose negatively affected transport of both D-glucose and D-xylose. Determination of kinetic properties of the mutant transporters revealed that Gal2-N376F had the highest affinity for D-xylose, along with a moderate transport velocity, and had completely lost the ability to transport hexoses. These transporter versions should prove valuable for glucose-xylose cofermentation in lignocellulosic hydrolysates by Saccharomyces cerevisiae and other biotechnologically relevant organisms. Moreover, our data contribute to the mechanistic understanding of sugar transport because the decisive role of the conserved asparagine residue for determining sugar specificity has not been recognized before.
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