ArticleProceedings of the National Academy of Sciences of the United States of America2014
Functional characterization of flavobacteria rhodopsins reveals a unique class of light-driven chloride pump in bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 166 citations in OpenAlex.
- Mechanistic insights into NaThe Journal of biological chemistry · 2026Article
- Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota.Nature microbiology · 2025Article
- A Detailed View on the (Re)isomerization Dynamics in Microbial Rhodopsins Using Complementary Near-UV and IR Readouts.Angewandte Chemie (International ed. in English) · 2025Article
- Korea-Japan Biophysics Collaboration: A journey through microbial rhodopsin research.Biophysics and physicobiology · 2025Article
- Metagenomic Sequencing Elucidated the Microbial Diversity of Rearing Water Environments for Sichuan Taimen (Genes · 2024Article
- Light-driven anion-pumping rhodopsin with unique cytoplasmic anion-release mechanism.The Journal of biological chemistry · 2024Article
- Nanosecond Transient IR Spectroscopy of Halorhodopsin in Living Cells.Journal of the American Chemical Society · 2024Article
- A subgroup of light-driven sodium pumps with an additional Schiff base counterion.Nature communications · 2024Article
- Draft genome sequences of three rhodopsin possessingMicrobiology resource announcements · 2024Article
- Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean.Frontiers in microbiology · 2024Article
- Pangenome analysis provides insights into the genetic diversity, metabolic versatility, and evolution of the genusMicrobiology spectrum · 2023Article
- KInternational journal of molecular sciences · 2023Article
- A Proteorhodopsin-Related Photosensor Expands the Repertoire of Structural Motifs Employed by Sensory Rhodopsins.The journal of physical chemistry. B · 2023Article
- Flotillin-associated rhodopsin (FArhodopsin), a widespread paralog of proteorhodopsin in aquatic bacteria with streamlined genomes.mSystems · 2023Article
- Potassium-selective channelrhodopsins.Biophysics and physicobiology · 2023Article
- Ion-transporting mechanism in microbial rhodopsins: Mini-review relating to the session 5 at the 19th International Conference on Retinal Proteins.Biophysics and physicobiology · 2023Article
- Biophysical characterization of microbial rhodopsins with DSE motif.Biophysics and physicobiology · 2023Article
- Convergent evolution of animal and microbial rhodopsins.RSC advances · 2023Review
- Structural insights into light-driven anion pumping in cyanobacteria.Nature communications · 2022Article
- Mutations conferring SOScientific reports · 2022Article
20 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light-activated, ion-pumping rhodopsins are broadly distributed among many different bacteria and archaea inhabiting the photic zone of aquatic environments. Bacterial proton- or sodium-translocating rhodopsins can convert light energy into a chemiosmotic force that can be converted into cellular biochemical energy, and thus represent a widespread alternative form of photoheterotrophy. Here we report that the genome of the marine flavobacterium Nonlabens marinus S1-08(T) encodes three different types of rhodopsins: Nonlabens marinus rhodopsin 1 (NM-R1), Nonlabens marinus rhodopsin 2 (NM-R2), and Nonlabens marinus rhodopsin 3 (NM-R3). Our functional analysis demonstrated that NM-R1 and NM-R2 are light-driven outward-translocating H(+) and Na(+) pumps, respectively. Functional analyses further revealed that the light-activated NM-R3 rhodopsin pumps Cl(-) ions into the cell, representing the first chloride-pumping rhodopsin uncovered in a marine bacterium. Phylogenetic analysis revealed that NM-R3 belongs to a distinct phylogenetic lineage quite distant from archaeal inward Cl(-)-pumping rhodopsins like halorhodopsin, suggesting that different types of chloride-pumping rhodopsins have evolved independently within marine bacterial lineages. Taken together, our data suggest that similar to haloarchaea, a considerable variety of rhodopsin types with different ion specificities have evolved in marine bacteria, with individual marine strains containing as many as three functionally different rhodopsins.
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