ArticleProceedings of the National Academy of Sciences of the United States of America2014
Proteomic analysis of Vibrio cholerae outer membrane vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
What it found
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Who cites it
106 citing papers in PubMed.
- Article
- Host Factor Induced Bacterial Extracellular Vesicles Promote Horizontal Gene Transfer in Vibrio cholerae.Journal of extracellular vesicles · 2026Article
- Engineering Microbial Particles for Next-Generation Biomedical Platforms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Crystal structure ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Outer membrane vesicles inMicrobial cell (Graz, Austria) · 2026Article
- Article
- Deciphering the immunomodulatory cross-talk: Bacterial extracellular vesicles in gut homeostasis.Virulence · 2025Review
- HtrA Contributes to Biofilm Formation inMicroorganisms · 2025Article
- Outer Membrane Vesicles as a Versatile Platform for Vaccine Development: Engineering Strategies, Applications and Challenges.Journal of extracellular vesicles · 2025Review
- Convergent mechanisms of epithelial cell structure manipulation by intestinal pathogens.PLoS pathogens · 2025Review
- Lipid a remodeling modulates outer membrane vesicle biogenesis byJournal of bacteriology · 2025Article
- Proteomic analysis of outer membrane vesicles derived from the type A5 Strain ofFrontiers in cellular and infection microbiology · 2025Article
- Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis.Frontiers in immunology · 2025Review
- Bacterial Extracellular Vesicles (BEVs) Derived FromInternational journal of biomaterials · 2025Article
- Bacterial extracellular vesicle: A non-negligible component in biofilm life cycle and challenges in biofilm treatments.Biofilm · 2024Review
- Molecular Mechanisms of Biofilm Formation inAntibiotics (Basel, Switzerland) · 2024Review
- An in-depth exploration of the multifaceted roles of EVs in the context of pathogenic single-cell microorganisms.Microbiology and molecular biology reviews : MMBR · 2024Review
- Article
- The activity of the quorum sensing regulator HapR is modulated by the bacterial extracellular vesicle (BEV)-associated protein ObfA of Vibrio cholerae.Journal of extracellular vesicles · 2024Article
- Extracellular Vesicles fromJournal of fungi (Basel, Switzerland) · 2024Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Outer membrane vesicles (OMVs) produced by Gram-negative bacteria provide an interesting research material for defining cell-envelope proteins without experimental cell disruption. OMVs are also promising immunogenic platforms and may play important roles in bacterial survival and pathogenesis. We used in-solution trypsin digestion coupled to mass spectrometry to identify 90 proteins present in OMVs of Vibrio cholerae when grown under conditions that activate the TCP pilus virulence regulatory protein (ToxT) virulence regulon. The ToxT expression profile and potential contribution to virulence of these proteins were assessed using ToxT and in vivo RNA-seq, Tn-seq, and cholera stool proteomic and other genome-wide data sets. Thirteen OMV-associated proteins appear to be essential for cell growth, and therefore may represent antibacterial drug targets. Another 12 nonessential OMV proteins, including DegP protease, were required for intestinal colonization in rabbits. Comparative proteomics of a degP mutant revealed the importance of DegP in the incorporation of nine proteins into OMVs, including ones involved in biofilm matrix formation and various substrates of the type II secretion system. Taken together, these results suggest that DegP plays an important role in determining the content of OMVs and also affects phenotypes such as intestinal colonization, proper function of the type II secretion system, and formation of biofilm matrix.
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