ArticleJournal of bacteriology2014
Genetic dissection of T4 lysis.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- The Lysis cassette of jumbophage PhiKZ.Scientific reports · 2026Article
- The Lysis Cassette of Jumbophage phiKZ.bioRxiv : the preprint server for biology · 2025Article
- The short cytoplasmic region of phage T4 holin is essential for the transition from impermeable membrane protein complexes to permeable pores.Frontiers in microbiology · 2025Article
- Characterization of holins, the membrane proteins of coliphage ASEC2201: a genomewideFrontiers in microbiology · 2025Article
- Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications.Protein and peptide letters · 2024Review
- Characterization of vB_ValM_PVA8, a broad-host-range bacteriophage infectingFrontiers in microbiology · 2023Article
- Characterization and Genomic Analysis of a Bacteriophage with Potential in Lysing Vibrio alginolyticusViruses · 2022Article
- Prediction and identification of new type holin protein ofFood science and biotechnology · 2022Article
- Endolysin Regulation in Phage Mu Lysis.mBio · 2022Article
- The Structural Basis of T4 Phage Lysis Control: DNA as the Signal for Lysis Inhibition.Journal of molecular biology · 2020Article
- Structural Dynamics and Topology of the Inactive Form of SThe journal of physical chemistry. B · 2020Article
- Look Who's Talking: T-Even Phage Lysis Inhibition, the Granddaddy of Virus-Virus Intercellular Communication Research.Viruses · 2019Review
- Commentary: A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision.Frontiers in microbiology · 2019Article
- Phage-Antibiotic Synergy via Delayed Lysis.Applied and environmental microbiology · 2018Article
- A Cytoplasmic Antiholin Is Embedded In Frame with the Holin in a Lactobacillus fermentum Bacteriophage.Applied and environmental microbiology · 2018Article
- The Last r Locus Unveiled: T4 RIII Is a Cytoplasmic Antiholin.Journal of bacteriology · 2016Article
- Bacteriophage secondary infection.Virologica Sinica · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). This holin topology is different from those of the well-studied holins S105 (3 TMDs; N(out)-C(in)) of the coliphage lambda and S68 (2 TMDs; N(in)-C(in)) of the lambdoid phage 21. Here, we used random mutagenesis to construct a library of lysis-defective alleles of t to discern residues and domains important for holin function and for the inhibition of lysis by the T4 antiholin, RI. The results show that mutations in all 3 topological domains (N-terminal cytoplasmic, TMD, and C-terminal periplasmic) can abrogate holin function. Additionally, several lysis-defective alleles in the C-terminal domain are no longer competent in binding RI. Taken together, these results shed light on the roles of the previously uncharacterized N-terminal and C-terminal domains in lysis and its real-time regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.