ArticlePlant physiology2014
Identification and Characterization of Maize floury4 as a Novel Semidominant Opaque Mutant That Disrupts Protein Body Assembly.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 69 citations in OpenAlex.
- Meta-QTLs and candidate genes for kernel protein content in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Modulating Purothionin Accumulation and Signal Peptide Cleavage Fine-Tunes Wheat Flour Gluten Properties to Improve Cookie-Making Quality.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Analysis of candidate genes identified via genome-wide association analysis of sugar-related traits in maize kernels.The plant genome · 2025Article
- Filamentous Temperature-Sensitive Z Protein J175 Regulates Maize Chloroplasts' and Amyloplasts' Division and Development.Plants (Basel, Switzerland) · 2025Article
- Maize big embryo 6 reveals roles of plastidial and cytosolic prephenate aminotransferases in seed and plant development.The Plant cell · 2025Article
- Endosperm cell death: roles and regulation in angiosperms.Journal of experimental botany · 2024Review
- Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transcriptomics and starch biosynthesis analysis in leaves and developing seeds of mung bean provide a basis for genetic engineering of starch composition and seed quality.Frontiers in plant science · 2024Article
- A translatome-transcriptome multi-omics gene regulatory network reveals the complicated functional landscape of maize.Genome biology · 2023Article
- Genome-wide association analysis of kernel nutritional quality in two natural maize populations.Molecular breeding : new strategies in plant improvement · 2023Article
- A Systemic Investigation of Genetic Architecture and Gene Resources Controlling Kernel Size-Related Traits in Maize.International journal of molecular sciences · 2023Article
- Unprocessed wheat γ-gliadin reduces gluten accumulation associated with the endoplasmic reticulum stress and elevated cell death.The New phytologist · 2022Article
- Endosperm-specific accumulation of human α-lactalbumin increases seed lysine content in maize.Plant cell reports · 2022Article
- Article
- Dynamic Transcriptome-Based Weighted Gene Co-expression Network Analysis Reveals Key Modules and Hub Genes Associated With the Structure and Nutrient Formation of Endosperm for Wax Corn.Frontiers in plant science · 2022Article
- StresSeed: The Unfolded Protein Response During Seed Development.Frontiers in plant science · 2022Review
- Genetic basis of maize kernel protein content revealed by high-density bin mapping using recombinant inbred lines.Frontiers in plant science · 2022Article
- Trihelix Transcription FactorInternational journal of molecular sciences · 2021Article
- Genome-wide association studies of grain quality traits in maize.Scientific reports · 2021Article
- Identification and fine mapping of a recessive gene controlling zebra leaf phenotype in maize.Molecular breeding : new strategies in plant improvement · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zeins are the major seed storage proteins in maize (Zea mays). They are synthesized on the endoplasmic reticulum (ER) and deposited into protein bodies. Failure of signal peptide cleavage from zeins can cause an opaque endosperm in the mature kernel; however, the cellular and molecular mechanisms responsible for this phenotype are not fully understood. In this study, we report the cloning and characterization of a novel, semidominant opaque mutant, floury4 (fl4). fl4 is caused by a mutated z1A 19-kD α-zein with defective signal peptide cleavage. Zein protein bodies in fl4 endosperm are misshapen and aggregated. Immunolabeling analysis indicated that fl4 participates in the assembly of zeins into protein bodies, disrupting their proper spatial distribution. ER stress is stimulated in fl4 endosperm, as illustrated by dilated rough ER and markedly up-regulated binding protein content. Further analysis confirmed that several ER stress pathways are induced in fl4 endosperm, including ER-associated degradation, the unfolded protein response, and translational suppression by the phosphorylation of eukaryotic translational initiation factor2 α-subunit. Programmed cell death is also elevated, corroborating the intensity of ER stress in fl4. These results provide new insights into cellular responses caused by storage proteins with defective signal peptides.
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Registered trials
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