ArticleMolecular biology reports2014
An insight into the genes involved in secoiridoid biosynthesis in Gentiana macrophylla by RNA-seq.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Gentianaceae Family-Derived Bioactive Compounds-Therapeutic Values and Supporting Role in Inflammation and Detoxification.Nutrients · 2025Review
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- Comparative transcriptome analyses of three Gentiana species provides signals for the molecular footprints of selection effects and the phylogenetic relationships.Molecular genetics and genomics : MGG · 2023Article
- Article
- De novo genome assembly of the medicinal plant Gentiana macrophylla provides insights into the genomic evolution and biosynthesis of iridoids.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- De Novo Transcriptome Analysis Reveals Flowering-Related Genes That Potentially Contribute to Flowering-Time Control in the Japanese Cultivated GentianInternational journal of molecular sciences · 2022Article
- The chromosome-level genome assembly of Gentiana dahurica (Gentianaceae) provides insights into gentiopicroside biosynthesis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- Analysis ofInternational journal of molecular sciences · 2019Article
- Comparative transcriptome analysis to identify putative genes involved in thymol biosynthesis pathway in medicinal plant Trachyspermum ammi L.Scientific reports · 2018Article
- Comparative Chloroplast Genome Analyses of Species inInternational journal of molecular sciences · 2018Article
- Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2017Article
- Deep sequencing and transcriptome analyses to identify genes involved in secoiridoid biosynthesis in the Tibetan medicinal plant Swertia mussotii.Scientific reports · 2017Article
- Comprehensive Quality Assessment Based Specific Chemical Profiles for Geographic and Tissue Variation inFrontiers in chemistry · 2017Article
- Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla.Frontiers in plant science · 2016Article
- Transcriptional Responses and Gentiopicroside Biosynthesis in Methyl Jasmonate-Treated Gentiana macrophylla Seedlings.PloS one · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dried root of Gentiana macrophylla is a well-known traditional Chinese herbal medicine for treating jaundice, hepatitis, and stomachic and choleretic ailments. However, natural sources are now in short supply. A lack of information about its genetic background has been a great hindrance to producing its active constituents via genetic engineering. We performed RNA-seq to obtain 42,918 unigenes (average length = 667 bp) in its transcriptome. Of these, 32,141 (74.89 %) were annotated and 2,339 unigenes were assigned to secondary-metabolite pathways. In all, 114 putative unigenes involved in secoiridoid biosynthesis were identified in our transcriptome library. A Blast X search against the Arabidopsis gene regulatory information server showed that 4,413 unigenes are homologous to transcription factor genes from Arabidopsis. Organ-specific genes and candidate gene expression profiles were also investigated with digital gene expression technology. Quantitative PCR was used to verify the expression patterns of several novel transcripts involved in secoiridoid biosynthesis. Our results not only enrich the gene resource but will also benefit research into the molecular genetics and functional genomics of this species.
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