ArticleNature protocols2014
Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Geminivirus-Derived Vectors as Tools for Functional Genomics.Frontiers in microbiology · 2022Review
- RNA Viral Vectors for Accelerating Plant Synthetic Biology.Frontiers in plant science · 2021Article
- Localization of Tobacco Yellow Dwarf Virus Replication Using the In Plant Activation (INPACT) Expression Platform.Viruses · 2020Article
- Screening of a multi-virus resistant RNAi construct in cowpea through transient vacuum infiltration method.Virusdisease · 2019Article
- Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification.Viruses · 2018Review
- Distinct domains of the AVRPM3The New phytologist · 2018Article
- Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform.Plant biotechnology journal · 2018Article
- Plant Virus Expression Vectors: A Powerhouse for Global Health.Biomedicines · 2017Review
- An Improved Syringe Agroinfiltration Protocol to Enhance Transformation Efficiency by Combinative Use of 5-Azacytidine, Ascorbate Acid and Tween-20.Plants (Basel, Switzerland) · 2017Article
- Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics.Current genomics · 2016Article
- Design of virus-based nanomaterials for medicine, biotechnology, and energy.Chemical Society reviews · 2016Review
- Towards engineering carboxysomes into C3 plants.The Plant journal : for cell and molecular biology · 2016Article
- Cotton Leaf Curl Multan Betasatellite DNA as a Tool to Deliver and Express the Human B-Cell Lymphoma 2 (Bcl-2) Gene in Plants.Molecular biotechnology · 2016Article
- Multiple Avirulence Loci and Allele-Specific Effector Recognition Control the Pm3 Race-Specific Resistance of Wheat to Powdery Mildew.The Plant cell · 2015Article
- Current status of viral expression systems in plants and perspectives for oral vaccines development.Plant molecular biology · 2015Review
- Review
- Inducible resistance to maize streak virus.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-based transgene expression systems have become particularly valuable for recombinant protein production in plants. The dual-module in-plant activation (INPACT) expression platform consists of a uniquely designed split-gene cassette incorporating the cis replication elements of Tobacco yellow dwarf geminivirus (TYDV) and an ethanol-inducible activation cassette encoding the TYDV Rep and RepA replication-associated proteins. The INPACT system is essentially tailored for recombinant protein production in stably transformed plants and provides both inducible and high-level transient transgene expression with the potential to be adapted to diverse crop species. The construction of a novel split-gene cassette, the inducible nature of the system and the ability to amplify transgene expression via rolling-circle replication differentiates this system from other DNA- and RNA-based virus vector systems used for stable or transient recombinant protein production in plants. Here we provide a detailed protocol describing the design and construction of a split-gene INPACT cassette, and we highlight factors that may influence optimal activation and amplification of gene expression in transgenic plants. By using Nicotiana tabacum, the protocol takes 6-9 months to complete, and recombinant proteins expressed using INPACT can accumulate to up to 10% of the leaf total soluble protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.