ArticleNature methods2014
Fluorescence nanoscopy by polarization modulation and polarization angle narrowing.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- SPIFFI enables single-shot super-resolution and multidimensional imaging.Nature methods · 2026Article
- Polarized detection sharpens optical live-cell fluorescence imaging.Nature methods · 2026Article
- Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence.Nanophotonics (Berlin, Germany) · 2025Article
- Unveiling Exciton-Plasmon Polariton Coupling Regions via Polarization-Enhanced Optical Nanoscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- High contrast fluorescence polarization microscopy through double tagged photoswitchable fluorescent proteins.Npj imaging · 2025Article
- Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.Physical review letters · 2025Article
- Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- Super-resolution imaging for the detection of low-energy ion tracks in fine-grained nuclear emulsions.Scientific reports · 2023Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- Fluorescence polarization modulation super-resolution imaging provides refined dynamics orientation processes in biological samples.Light, science & applications · 2022Article
- Article
- Split-HaloTag imaging assay for sophisticated microscopy of protein-protein interactionsPlant communications · 2021Article
- A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging.Microscopy (Oxford, England) · 2021Article
- Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts.International journal of molecular sciences · 2021Review
- Article
- Selected tools to visualize membrane interactions.European biophysics journal : EBJ · 2021Review
- High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes.Nature communications · 2020Article
- Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging.Journal of the Optical Society of America. A, Optics, image science, and vision · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When excited with rotating linear polarized light, differently oriented fluorescent dyes emit periodic signals peaking at different times. We show that measurement of the average orientation of fluorescent dyes attached to rigid sample structures mapped to regularly defined (50 nm)(2) image nanoareas can provide subdiffraction resolution (super resolution by polarization demodulation, SPoD). Because the polarization angle range for effective excitation of an oriented molecule is rather broad and unspecific, we narrowed this range by simultaneous irradiation with a second, de-excitation, beam possessing a polarization perpendicular to the excitation beam (excitation polarization angle narrowing, ExPAN). This shortened the periodic emission flashes, allowing better discrimination between molecules or nanoareas. Our method requires neither the generation of nanometric interference structures nor the use of switchable or blinking fluorescent probes. We applied the method to standard wide-field microscopy with camera detection and to two-photon scanning microscopy, imaging the fine structural details of neuronal spines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.