ArticlePloS one2014
Secretion of Antonospora (Paranosema) locustae proteins into infected cells suggests an active role of microsporidia in the control of host programs and metabolic processes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 62 citations in OpenAlex.
- Bioinformatics analysis of the Microsporidia sp. MB genome: a malaria transmission-blocking symbiont of the Anopheles arabiensis mosquito.BMC genomics · 2024Article
- Treatment of MicrosporidiumMicroorganisms · 2024Article
- Molecular and biochemical changes in Locusta migratoria (Orthoptera: Acrididae) infected with Paranosema locustae.Journal of insect science (Online) · 2023Article
- Article
- A Putative TRAPα Protein of MicrosporidiaJournal of fungi (Basel, Switzerland) · 2023Article
- Structural and Functional Annotation of Hypothetical Proteins from the Microsporidia SpeciesInternational journal of molecular sciences · 2023Article
- Nosema apis and N. ceranae Infection in Honey bees: A Model for Host-Pathogen Interactions in Insects.Experientia supplementum (2012) · 2022Article
- Suppression of yolk formation, oviposition and egg quality of locust (Frontiers in immunology · 2022Article
- Article
- Evolution of microsporidia: An extremely successful group of eukaryotic intracellular parasites.PLoS pathogens · 2020Article
- Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper.Journal of insect science (Online) · 2019Article
- Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.Parasitology research · 2019Article
- Encephalitozoon cuniculi and Vittaforma corneae (Phylum Microsporidia) inhibit staurosporine-induced apoptosis in human THP-1 macrophages in vitro.Parasitology · 2019Article
- The invasive cell coat at the microsporidian Trachipleistophora hominis-host cell interface contains secreted hexokinases.MicrobiologyOpen · 2019Article
- Article
- A Novel Spore Wall Protein from Antonospora locustae (Microsporidia: Nosematidae) Contributes to Sporulation.The Journal of eukaryotic microbiology · 2017Article
- Identification of microsporidia host-exposed proteins reveals a repertoire of rapidly evolving proteins.Nature communications · 2017Article
- Transcriptome sequencing and characterization of ungerminated and germinated spores of Nosema bombycis.Acta biochimica et biophysica Sinica · 2016Article
- Microsporidia-host interactions.Current opinion in microbiology · 2015Review
- Contrasting host-pathogen interactions and genome evolution in two generalist and specialist microsporidian pathogens of mosquitoes.Nature communications · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular tools of the intracellular protozoan pathogens Apicomplexa and Kinetoplastida for manipulation of host cell machinery have been the focus of investigation for approximately two decades. Microsporidia, fungi-related microorganisms forming another large group of obligate intracellular parasites, are characterized by development in direct contact with host cytoplasm (the majority of species), strong minimization of cell machinery, and acquisition of unique transporters to exploit host metabolic system. All the aforementioned features are suggestive of the ability of microsporidia to modify host metabolic and regulatory pathways. Seven proteins of the microsporidium Antonospora (Paranosema) locustae with predicted signal peptides but without transmembrane domains were overexpressed in Escherichia coli. Western-blot analysis with antibodies against recombinant products showed secretion of parasite proteins from different functional categories into the infected host cell. Secretion of parasite hexokinase and α/β-hydrolase was confirmed by immunofluorescence microscopy. In addition, this method showed specific accumulation of A. locustae hexokinase in host nuclei. Expression of hexokinase, trehalase, and two leucine-rich repeat proteins without any exogenous signal peptide led to their secretion in the yeast Pichia pastoris. In contrast, α/β-hydrolase was not found in the culture medium, though a significant amount of this enzyme accumulated in the yeast membrane fraction. These results suggest that microsporidia possess a broad set of enzymes and regulatory proteins secreted into infected cells to control host metabolic processes and molecular programs.
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