ArticlePloS one2014
Three amino acid residues bind corn odorants to McinOBP1 in the polyembryonic endoparasitoid of Macrocentrus cingulum Brischke.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 22 citations in OpenAlex.
- SNMPs Function as Molecular Bridges between OBPs and ORs in the Olfactory Recognition of Clostera Retitura (Walker) (Lepidoptera: Notodontidae).Journal of chemical ecology · 2025Article
- Molecular Characterization of Chemosensory Protein (CSP) Genes and the Involvement ofInternational journal of molecular sciences · 2024Article
- Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata.Scientific reports · 2021Article
- Molecular characterization and functional differentiation of three pheromone-binding proteins from Tryporyza intacta.Scientific reports · 2018Article
- Molecular and Functional Characterization of pheromone binding protein 1 from the Oriental Fruit Moth, Grapholita molesta (Busck).Scientific reports · 2018Article
- Binding Specificity of Two PBPs in the Yellow Peach MothFrontiers in physiology · 2018Article
- C-terminus Methionene Specifically Involved in Binding Corn Odorants to Odorant Binding Protein4 inFrontiers in physiology · 2017Article
- Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies.BMC evolutionary biology · 2016Article
- Gene set of chemosensory receptors in the polyembryonic endoparasitoid Macrocentrus cingulum.Scientific reports · 2016Article
- Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae).International journal of biological sciences · 2015Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Odorant binding proteins (OBPs) play a central role in transporting odorant molecules from the sensillum lymph to olfactory receptors to initiate behavioral responses. In this study, the OBP of Macrocentrus cingulum McinOBP1 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR experiments indicate that the McinOBP1 is expressed mainly in adult antennae, with expression levels differing by sex. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the McinOBP1 can bind green-leaf volatiles, including aldehydes and terpenoids, but also can bind aliphatic alcohols with good affinity, in the order trans-2-nonenal>cis-3-hexen-1-ol>trans-caryophelle, suggesting a role of McinOBP1 in general odorant chemoreception. We chose those three odorants for further homology modeling and ligand docking based on their binding affinity. The Val58, Leu62 and Glu130 are the key amino acids in the binding pockets that bind with these three odorants. The three mutants, Val58, Leu62 and Glu130, where the valine, leucine and glutamic residues were replaced by alanine, proline and alanine, respectively; showed reduced affinity to these odorants. This information suggests, Val58, Leu62 and Glu130 are involved in the binding of these compounds, possibly through the specific recognition of ligands that forms hydrogen bonds with the ligands functional groups.
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