ArticlePloS one2014
Reconstruction of auto-tissue-engineered lamellar cornea by dynamic culture for transplantation: a rabbit model.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02148016 (Corneal Epithelium Repair and Therapy Using Autologous Limbal Stem Cell Transplantation), which is not on this map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Corneal Epithelium Repair and Therapy Using Autologous Limbal Stem Cell Transplantation
Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Corneal Epithelial Tissue Engineering Strategy Based on Cell Viability Optimization: A Review and Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- Article
- Hydrogels derived from acellular porcine corneal stroma enhance corneal wound healing.Acta biomaterialia · 2021Article
- Paracrine activity of adipose derived stem cells on limbal epithelial stem cells.Scientific reports · 2021Article
- Corneal pain and experimental model development.Progress in retinal and eye research · 2019Review
- Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects.International journal of molecular sciences · 2019Article
- In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers.Scientific reports · 2017Article
- Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system.Cytotechnology · 2016Article
- Limbal Stem Cell Deficiency: Current Treatment Options and Emerging Therapies.Stem cells international · 2016Review
- Progress in corneal wound healing.Progress in retinal and eye research · 2015Review
- Corneal stem cells and tissue engineering: Current advances and future perspectives.World journal of stem cells · 2015Review
- Importance of the stem cell microenvironment for ophthalmological cell-based therapy.World journal of stem cells · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To construct an auto-tissue-engineered lamellar cornea (ATELC) for transplantation, based on acellular porcine corneal stroma and autologous corneal limbal explants, a dynamic culture process, which composed of a submersion culture, a perfusion culture and a dynamic air-liquid interface culture, was performed using appropriate parameters. The results showed that the ATELC-Dynamic possessed histological structure and DNA content that were similar to native lamellar cornea (NLC, p>0.05). Compared to NLC, the protein contents of zonula occludens-1, desmocollin-2 and integrin β4 in ATELC-Dynamic reached 93%, 89% and 73%, respectively. The basal cells of ATELC-Dynamic showed a better differentiation phenotype (K3-, P63+, ABCG2+) compared with that of ATELC in static air-lift culture (ATELC-Static, K3+, P63-, ABCG2-). Accordingly, the cell-cloning efficiency of ATELC-Dynamic (9.72±3.5%) was significantly higher than that of ATELC-Static (2.13±1.46%, p<0.05). The levels of trans-epithelial electrical resistance, light transmittance and areal modulus variation in ATELC-Dynamic all reached those of NLC (p>0.05). Rabbit lamellar keratoplasty showed that the barrier function of ATELC-Dynamic was intact, and there were no signs of epithelial shedding or neovascularization. Furthermore, the ATELC-Dynamic group had similar optical properties and wound healing processes compared with the NLC group. Thus, the sequential dynamic culture process that was designed according to corneal physiological characteristics could successfully reconstruct an auto-lamellar cornea with favorable morphological characteristics and satisfactory physiological function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.