ArticleApplied and environmental microbiology2014
Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Randomised clinical trial: gut microbiome biomarkers are associated with clinical response to a low FODMAP diet in children with the irritable bowel syndrome.Alimentary pharmacology & therapeutics · 2015Trial
- A CRISPRi Gene Regulation System for Bifidobacteria.Microbial biotechnology · 2025Article
- Article
- Gene-trait matching amongFrontiers in microbiology · 2025Article
- Inducible directed evolution of complex phenotypes in bacteria.Nucleic acids research · 2022Article
- Plant Glycan Metabolism by Bifidobacteria.Frontiers in microbiology · 2021Review
- The local transcriptional regulators SacR1 and SacR2 act as repressors of fructooligosaccharides metabolism in Lactobacillus plantarum.Microbial cell factories · 2020Article
- Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators.Scientific reports · 2019Article
- Bifidobacterium breve UCC2003 Employs Multiple Transcriptional Regulators To Control Metabolism of Particular Human Milk Oligosaccharides.Applied and environmental microbiology · 2018Article
- The Sortase-Dependent Fimbriome of the Genus Bifidobacterium: Extracellular Structures with Potential To Modulate Microbe-Host Dialogue.Applied and environmental microbiology · 2017Article
- Mechanistic Study of Utilization of Water-Insoluble Saccharomyces cerevisiae Glucans by Bifidobacterium breve Strain JCM1192.Applied and environmental microbiology · 2017Article
- Genome-Wide Search for Genes Required for Bifidobacterial Growth under Iron-Limitation.Frontiers in microbiology · 2017Article
- Glycosulfatase-Encoding Gene Cluster in Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2016Article
- Characterization of DNA Binding Sites of RokB, a ROK-Family Regulator from Streptomyces coelicolor Reveals the RokB Regulon.PloS one · 2016Article
- Bifidobacteria and Their Role as Members of the Human Gut Microbiota.Frontiers in microbiology · 2016Review
- Transcriptional Regulation of Carbohydrate Utilization Pathways in the Bifidobacterium Genus.Frontiers in microbiology · 2016Article
- Metabolism of Fructooligosaccharides in Lactobacillus plantarum ST-III via Differential Gene Transcription and Alteration of Cell Membrane Fluidity.Applied and environmental microbiology · 2015Article
- Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium.BMC microbiology · 2014Article
- Experimental determination and characterization of the gap promoter of Bifidobacterium bifidum S17.Bioengineered · 2014Article
- Milk glycan metabolism by intestinal bifidobacteria: insights from comparative genomics.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Bifidobacterium are commonly found in the gastrointestinal tracts of mammals, including humans, where their growth is presumed to be dependent on various diet- and/or host-derived carbohydrates. To understand transcriptional control of bifidobacterial carbohydrate metabolism, we investigated two genetic carbohydrate utilization clusters dedicated to the metabolism of raffinose-type sugars and melezitose. Transcriptomic and gene inactivation approaches revealed that the raffinose utilization system is positively regulated by an activator protein, designated RafR. The gene cluster associated with melezitose metabolism was shown to be subject to direct negative control by a LacI-type transcriptional regulator, designated MelR1, in addition to apparent indirect negative control by means of a second LacI-type regulator, MelR2. In silico analysis, DNA-protein interaction, and primer extension studies revealed the MelR1 and MelR2 operator sequences, each of which is positioned just upstream of or overlapping the correspondingly regulated promoter sequences. Similar analyses identified the RafR binding operator sequence located upstream of the rafB promoter. This study indicates that transcriptional control of gene clusters involved in carbohydrate metabolism in bifidobacteria is subject to conserved regulatory systems, representing either positive or negative control.
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