ArticleGenes2012
siRNAs Trigger Efficient Silencing of a Parasitism Gene in Plant Parasitic Root-Knot Nematodes.
Article in Genes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Transgenic East African Highland Banana Plants Are Protected againstInternational journal of molecular sciences · 2023Article
- A novel pine wood nematode effector, BxSCD1, suppresses plant immunity and interacts with an ethylene-forming enzyme in pine.Molecular plant pathology · 2021Article
- Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants.International journal of molecular sciences · 2021Review
- RNA interference of an orthologue of Dicer of Meloidogyne incognita alludes to the gene's importance in nematode development.Scientific reports · 2021Article
- Analyses of the Root-Knot Nematode (Pathogens (Basel, Switzerland) · 2020Article
- Attempt to Silence Genes of the RNAi Pathways of the Root-Knot Nematode,Frontiers in plant science · 2020Article
- In Vitro Acquisition of Specific Small Interfering RNAs Inhibits the Expression of Some Target Genes in the Plant EctoparasiteInternational journal of molecular sciences · 2019Article
- Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems.Scientific reports · 2019Article
- Cross-kingdom RNA trafficking and environmental RNAi-nature's blueprint for modern crop protection strategies.Current opinion in microbiology · 2018Review
- Article
- Identification of novel target genes for safer and more specific control of root-knot nematodes from a pan-genome mining.PLoS pathogens · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expanding genomic data on plant pathogens open new perspectives for the development of specific and environment friendly pest management strategies based on the inhibition of parasitism genes that are essential for the success of infection. Identifying such genes relies on accurate reverse genetics tools and the screening of pathogen knock-down phenotypes. Root-knot nematodes are major cosmopolitan crop pests that feed on a wide range of host plants. Small interfering RNAs (siRNAs) would provide a powerful tool for reverse genetics of nematode parasitism genes provided that they could (1) target genes expressed in inner tissues of infective nematodes and (2) target genes expressed during parasitism. In this study, we show that siRNAs can access inner tissues of the infective juveniles during soaking and accumulate in the esophagus, amphidial pouches and related neurons of the nematode. We provide evidence that siRNAs can trigger knock-down of the parasitism gene Mi-CRT, a calreticulin gene expressed in the esophageal glands of Meloidogyne incognita. Mi-CRT knock-down in infective juveniles affected nematode virulence. However, Mi-CRT knock-down was not persistent after plant infection, indicating that siRNA-mediated RNAi is best suited for functional analysis of genes involved in pre-parasitic stages or in the early steps of infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.