ArticlePloS one2014
Myb transcription factors and light regulate sporulation in the oomycete Phytophthora infestans.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Transcriptional programs controlling sporulation in Phytophthora respond to nutrition, humidity, and cryptochrome-mediated light signals.PLoS pathogens · 2026Article
- Characterization ofJournal of fungi (Basel, Switzerland) · 2026Article
- Transient expression of fluorescent proteins and Cas nucleases inMicrobiology (Reading, England) · 2025Article
- Promoter Analysis and Dissection Using Reporter Genes, Comparative Genomics, and Gel Shift Assays in Phytophthora.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the MYB-R2R3 Gene Family in Black Pepper (International journal of molecular sciences · 2024Article
- Transcription factor binding specificities of the oomycete Phytophthora infestans reflect conserved and divergent evolutionary patterns and predict function.BMC genomics · 2024Article
- Transcriptome analysis reveals genes associated with late blight resistance in potato.Scientific reports · 2024Article
- A MYB-related transcription factor regulates effector gene expression in an oomycete pathogen.Molecular plant pathology · 2024Article
- Effect of Different Light Wavelengths onJournal of fungi (Basel, Switzerland) · 2023Article
- Tuning the Wavelength: Manipulation of Light Signaling to Control Plant Defense.International journal of molecular sciences · 2023Review
- Screening of wild species and transcriptome profiling to identify differentially regulated genes in response to late blight resistance in potato.Frontiers in plant science · 2023Article
- Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis.Microbial cell factories · 2022Article
- Identification of a CJournal of fungi (Basel, Switzerland) · 2022Article
- Signal and regulatory mechanisms involved in spore development ofFrontiers in microbiology · 2022Review
- Predicting Lifestyle from Positive Selection Data and Genome Properties in Oomycetes.Pathogens (Basel, Switzerland) · 2021Article
- Organize, Don't Agonize: Strategic Success ofMicroorganisms · 2020Review
- A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans.Molecular plant pathology · 2020Article
- G protein α subunit suppresses sporangium formation through a serine/threonine protein kinase in Phytophthora sojae.PLoS pathogens · 2020Article
- Comparative Transcriptome Analysis Revealed Genes Regulated by Histone Acetylation and Genes Related to Sex Hormone Biosynthesis inFrontiers in genetics · 2020Article
- Exchanges at the Plant-Oomycete Interface That Influence Disease.Plant physiology · 2019Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Life cycle progression in eukaryotic microbes is often influenced by environment. In the oomycete Phytophthora infestans, which causes late blight on potato and tomato, sporangia have been reported to form mostly at night. By growing P. infestans under different light regimes at constant temperature and humidity, we show that light contributes to the natural pattern of sporulation by delaying sporulation until the following dark period. However, illumination does not permanently block sporulation or strongly affect the total number of sporangia that ultimately form. Based on measurements of sporulation-induced genes such as those encoding protein kinase Pks1 and Myb transcription factors Myb2R1 and Myb2R3, it appears that most spore-associated transcripts start to rise four to eight hours before sporangia appear. Their mRNA levels oscillate with the light/dark cycle and increase with the amount of sporangia. An exception to this pattern of expression is Myb2R4, which is induced several hours before the other genes and declines after cultures start to sporulate. Transformants over-expressing Myb2R4 produce twice the number of sporangia and ten-fold higher levels of Myb2R1 mRNA than wild-type, and chromatin immunoprecipitation showed that Myb2R4 binds the Myb2R1 promoter in vivo. Myb2R4 thus appears to be an early regulator of sporulation. We attempted to silence eight Myb genes by DNA-directed RNAi, but succeeded only with Myb2R3, which resulted in suppressed sporulation. Ectopic expression studies of seven Myb genes revealed that over-expression frequently impaired vegetative growth, and in the case of Myb3R6 interfered with sporangia dormancy. We observed that the degree of silencing induced by a hairpin construct was correlated with its copy number, and ectopic expression was often unstable due to epigenetic silencing and transgene excision.
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