ArticleOsteoarthritis and cartilage2014
Connexin43 hemichannels mediate small molecule exchange between chondrocytes and matrix in biomechanically-stimulated temporomandibular joint cartilage.
Article in Osteoarthritis and cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Loss of connexin 43 in cartilage causes mitochondrial dysfunction and accelerates post-traumatic osteoarthritis progression.bioRxiv : the preprint server for biology · 2026Article
- Equine Models of Temporomandibular Joint Osteoarthritis: A Review of Feasibility, Biomarkers, and Molecular Signaling.Biomedicines · 2024Review
- Critical signaling molecules in the temporomandibular joint osteoarthritis under different magnitudes of mechanical stimulation.Frontiers in pharmacology · 2024Review
- Potential pathological and molecular mechanisms of temporomandibular joint osteoarthritis.Journal of dental sciences · 2023Review
- Natural compounds protect against the pathogenesis of osteoarthritis by mediating the NRF2/ARE signaling.Frontiers in pharmacology · 2023Review
- The role of mechanobiology in bone and cartilage model systems in characterizing initiation and progression of osteoarthritis.APL bioengineering · 2022Review
- Chondrocyte-derived exosomes promote cartilage calcification in temporomandibular joint osteoarthritis.Arthritis research & therapy · 2022Article
- Mechanosignalling in cartilage: an emerging target for the treatment of osteoarthritis.Nature reviews. Rheumatology · 2022Review
- Molecular signaling in temporomandibular joint osteoarthritis.Journal of orthopaedic translation · 2022Review
- Mechanotransduction pathways in the regulation of cartilage chondrocyte homoeostasis.Journal of cellular and molecular medicine · 2020Review
- MTORC1 coordinates the autophagy and apoptosis signaling in articular chondrocytes in osteoarthritic temporomandibular joint.Autophagy · 2020Article
- Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.International journal of molecular sciences · 2019Article
- Prevention of Injury-Induced Osteoarthritis in Rodent Temporomandibular Joint by Targeting Chondrocyte CaSR.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- TGF-β1 promotes gap junctions formation in chondrocytes via Smad3/Smad4 signalling.Cell proliferation · 2019Article
- Joint diseases: from connexins to gap junctions.Nature reviews. Rheumatology · 2017Review
- Connexin Hemichannels in Astrocytes: An Assessment of Controversies Regarding Their Functional Characteristics.Neurochemical research · 2017Review
- Role of connexins and pannexins during ontogeny, regeneration, and pathologies of bone.BMC cell biology · 2016Review
- Unilateral anterior crossbite induces aberrant mineral deposition in degenerative temporomandibular cartilage in rats.Osteoarthritis and cartilage · 2016Article
- Connexins and pannexins in the skeleton: gap junctions, hemichannels and more.Cellular and molecular life sciences : CMLS · 2015Review
- Physiological roles of connexins and pannexins in reproductive organs.Cellular and molecular life sciences : CMLS · 2015Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveConnexin (Cx) 43 hemichannels play a role in mechanotransduction. This study was undertaken in order to determine if Cx43 hemichannels were activated in rat temporomandibular joint (TMJ) chondrocytes under mechanical stimulation.
methodsSprague-Dawley rats were stimulated dental-mechanically. Cx43 expression in rat TMJ cartilage was determined with immunohistochemistry and real-time PCR, and Cx43 hemichannel opening was evaluated by the extra- and intracellular levels of prostaglandin E2 (PGE2). Both primary rat chondrocytes and ATDC5 cells were treated with fluid flow shear stress (FFSS) to induce hemichannel opening. The Cx43 expression level was then determined by real-time PCR or Western blotting, and the extent of Cx43 hemichannel opening was evaluated by measuring both PGE2 release and cellular dye uptake.
resultsCx43 expression and intra- and extracellular PGE2 levels were increased in mechanically-stimulated rat TMJ cartilage compared to the unstimulated control. The FFSS treatment increased Cx43 expression and induced Cx43 hemichannel opening in primary rat chondrocytes and ATDC5 cells indicated by enhanced PGE2 release and dye uptake. Furthermore, the Cx43 hemichannel opening could be blocked by the addition of 18β-glycyrrhetinic acid, a Cx channel inhibitor, Cx43-targeting siRNA, or by withdrawal of FFSS stimulation. The migration of cytosolic Cx43 protein to the plasma membrane in ATDC5 cells was still significant after 8 h post 2-h FFSS treatment, and the Cx43 protein level was still high at 48 h, which returned to control levels at 72 h after treatment.
conclusionCx43 hemichannels are activated and mediate small molecule exchange between TMJ chondrocytes and matrix under mechanical stimulation.
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Registered trials
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