ArticleBiophysical journal2014
Novel growth regime of MDCK II model tissues on soft substrates.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Capturing the mechanosensitivity of cell proliferation in models of epithelium.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Role of ZO-2 in Modulating JAM-A and γ-Actin Junctional Recruitment, Apical Membrane and Tight Junction Tension, and Cell Response to Substrate Stiffness and Topography.International journal of molecular sciences · 2024Article
- Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins.ACS nano · 2023Article
- FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking.PloS one · 2023Article
- Substrate Stiffness Determines the Establishment of Apical-Basal Polarization in Renal Epithelial Cells but Not in Tubuloid-Derived Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Measuring Nuclear Mechanics with Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- DNA damage alters nuclear mechanics through chromatin reorganization.Nucleic acids research · 2021Article
- Effect of Adhesion and Substrate Elasticity on Neutrophil Extracellular Trap Formation.Frontiers in immunology · 2019Article
- Active wetting of epithelial tissues.Nature physics · 2019Article
- A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells.Journal of visualized experiments : JoVE · 2018Article
- Mechanobiology of collective cell behaviours.Nature reviews. Molecular cell biology · 2017Review
- Emergent patterns of collective cell migration under tubular confinement.Nature communications · 2017Article
- A critical-like collective state leads to long-range cell communication in Dictyostelium discoideum aggregation.PLoS biology · 2017Article
- Limits of Applicability of the Voronoi Tessellation Determined by Centers of Cell Nuclei to Epithelium Morphology.Frontiers in physiology · 2016Article
- Mechanotransduction: use the force(s).BMC biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
It is well established that MDCK II cells grow in circular colonies that densify until contact inhibition takes place. Here, we show that this behavior is only typical for colonies developing on hard substrates and report a new growth phase of MDCK II cells on soft gels. At the onset, the new phase is characterized by small, three-dimensional droplets of cells attached to the substrate. When the contact area between the agglomerate and the substrate becomes sufficiently large, a very dense monolayer nucleates in the center of the colony. This monolayer, surrounded by a belt of three-dimensionally packed cells, has a well-defined structure, independent of time and cluster size, as well as a density that is twice the steady-state density found on hard substrates. To release stress in such dense packing, extrusions of viable cells take place several days after seeding. The extruded cells create second-generation clusters, as evidenced by an archipelago of aggregates found in a vicinity of mother colonies, which points to a mechanically regulated migratory behavior.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.