Evidence map›Paper›PMID 24702961›Full record

ArticleBiomaterials2014

The crucial role of mechanical heterogeneity in regulating follicle development and ovulation with engineered ovarian microtissue.

Jung Kyu Choi, Pranay Agarwal, Haishui Huang, Shuting Zhao, Xiaoming He

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed, 2 pooled it
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed, 2 syntheses or guidelines pooled it, 112 citations in OpenAlex.

  1. Pooled it
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  10. Observational
  11. Article
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  15. In Vitro Growth of Human Follicles: Current and Future Perspectives.International journal of molecular sciences · 2024
    Review
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  19. Article
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7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jung Kyu ChoiDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
Pranay AgarwalDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
Haishui HuangDorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.
Shuting ZhaoDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
Xiaoming HeDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA; Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA. Electronic address: he.429@osu.edu.
The Ohio State University · USLung Institute · US

Funding

Microfluidic encapsulation of ovarian follicles for biomimetic 3D culture andcryopreservationR01EB023632 · NIBIB · UNIV OF MARYLAND, COLLEGE PARK · PI HE, XIAOMING · 2017 to 2020
$1.6M
Microencapsulation of oocytes for low-CPA (cryoprotectant) vitrificationR01EB012108 · NIBIB · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI HE, XIAOMING · 2011 to 2014
$1.3M
NIBIB NIH HHS R01 EB012108NIBIB NIH HHS R01EB012108NIBIB NIH HHS R01 EB023632
6 · The paper itself

Abstract

Contemporary systems for in vitro culture of ovarian follicles do not recapitulate the mechanical heterogeneity in mammalian ovary. Here we report microfluidic generation of biomimetic ovarian microtissue for miniaturized three-dimensional (3D) culture of early secondary preantral follicles by using alginate (harder) and collagen (softer) to fabricate the ovarian cortical and medullary tissues, respectively. This biomimetic configuration greatly facilitates follicle development to antral stage. Moreover, it enables in vitro ovulation of cumulus-oocyte complex (COC) from the antral follicles in the absence of luteinizing hormone (LH) and epidermal growth factor (EGF) that are well accepted to be responsible for ovulation in contemporary literature. These data reveal the crucial role of mechanical heterogeneity in the mammalian ovary in regulating follicle development and ovulation. The biomimetic ovarian microtissue and the microfluidic technology developed in this study are valuable for improving in vitro culture of follicles to preserve fertility and for understanding the mechanism of follicle development and ovulation to facilitate the search of cures to infertility due to ovarian disorders.

Indexed as

AlginatesAnimalsFemaleGlucuronic AcidHexuronic AcidsOvarian FollicleOvaryOvulationPeromyscusTissue EngineeringAlginatesGlucuronic AcidHexuronic AcidsAlginateFollicleMechanobiologyMicrofluidicMicrotissueOvulation

Identifiers

PMID24702961
PMCPMC4016819
OpenAlexW1988752403

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.