ArticlePathogens and disease2014
The genetic basis of plasmid tropism between Chlamydia trachomatis and Chlamydia muridarum.
Article in Pathogens and disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 30 citations in OpenAlex.
- Calcium Chloride-Mediated Transformation of Chlamydia trachomatis and Other Chlamydia spp.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Transformation and allelic exchange inmBio · 2026Article
- Transformation and allelic exchange inbioRxiv : the preprint server for biology · 2026Article
- Development of shuttle vector-based transformation systems for veterinary and zoonotic chlamydiae.Microbiology spectrum · 2025Article
- A platform supporting generation and isolation of random transposon mutants inJournal of bacteriology · 2025Article
- Zoonotic and other veterinary chlamydiae - an update, the role of the plasmid and plasmid-mediated transformation.Pathogens and disease · 2024Review
- Article
- Recent advances in genetic systems in obligate intracellular human-pathogenic bacteria.Frontiers in cellular and infection microbiology · 2023Review
- A Minimal Replicon Enables Efficacious, Species-Specific Gene Deletion in Chlamydia and Extension of Gene Knockout Studies to the Animal Model of Infection Using Chlamydia muridarum.Infection and immunity · 2022Article
- Chlamydia repurposes the actin-binding protein EPS8 to disassemble epithelial tight junctions and promote infection.Cell host & microbe · 2022Article
- The growing repertoire of genetic tools for dissecting chlamydial pathogenesis.Pathogens and disease · 2021Article
- An endometrial organoid model of interactions betweenJournal of cell science · 2021Article
- Progress towards an inducible, replication-proficient transposon delivery vector forWellcome open research · 2021Article
- Polymorphic Membrane Protein 17G ofFrontiers in immunology · 2021Article
- Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci.mSphere · 2020Article
- The Nature and Extent of Plasmid Variation inMicroorganisms · 2020Article
- Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays.Molecular medicine reports · 2020Article
- Diversity in Chlamydial plasmids.PloS one · 2020Article
- Development of Transposon Mutagenesis for Chlamydia muridarum.Journal of bacteriology · 2019Article
- Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
The development of genetic transformation technology for Chlamydia trachomatis using its endogenous plasmid has recently been described. Chlamydia muridarum cannot be transformed by the C. trachomatis plasmid, indicating a barrier between chlamydial species. To determine which regions of the plasmid conferred the species specificity, we used the novel approach of transforming wild-type C. muridarum carrying the endogenous plasmid pNigg and forced recombination with the C. trachomatis vector pGFP::SW2 which carries the complete C. trachomatis plasmid (pSW2). Penicillin and chloramphenicol-resistant transformants expressing the green fluorescent protein were selected. Recovery of plasmids from these transformants showed they were recombinants. The differences between the pSW2 and pNigg allowed identification of the recombination breakpoints and showed that pGFP::SW2 had exchanged a ~ 1 kbp region with pNigg covering CDS 2. The recombinant plasmid (pSW2NiggCDS2) is maintained under antibiotic selection when transformed into plasmid-cured C. muridarum. The ability to select for recombinants in C. muridarum shows that the barrier is not at transformation, but at the level of plasmid replication or maintenance. Our studies show that CDS 2, together with adjoining sequences, is the main determinant of plasmid tropism.
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