ArticleJournal of biomolecular NMR2014
New 13C-detected experiments for the assignment of intrinsically disordered proteins.
Article in Journal of biomolecular NMR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- A call to order: Examining structured domains in biomolecular condensates.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2023Article
- Insight into polyproline II helical bundle stability in an antifreeze protein denatured state.Biophysical journal · 2022Article
- Conformational dynamics in the disordered region of human CPEB3 linked to memory consolidation.BMC biology · 2022Article
- Review
- Structural transitions in Orb2 prion-like domain relevant for functional aggregation in memory consolidation.The Journal of biological chemistry · 2020Article
- HACANCOi: a new HJournal of biomolecular NMR · 2020Article
- Advances in studying protein disorder with solid-state NMR.Solid state nuclear magnetic resonance · 2020Review
- Dispersion from CJournal of biomolecular NMR · 2020Article
- A CON-based NMR assignment strategy for pro-rich intrinsically disordered proteins with low signal dispersion: the C-terminal domain of histone H1.0 as a case study.Journal of biomolecular NMR · 2018Article
- A Mechanism of Calmodulin Modulation of the Human Cardiac Sodium Channel.Structure (London, England : 1993) · 2018Article
- Carbon and amide detect backbone assignment methods of a novel repeat protein from the staphylocoagulase in S. aureus.Biomolecular NMR assignments · 2017Article
- Quarterly intrinsic disorder digest (April-May-June, 2014).Intrinsically disordered proteins · 2017Article
- Direct ¹³C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA.Journal of biomolecular NMR · 2016Article
- Six- and seven-dimensional experiments by combination of sparse random sampling and projection spectroscopy dedicated for backbone resonance assignment of intrinsically disordered proteins.Journal of biomolecular NMR · 2015Article
- HN-NCA heteronuclear TOCSY-NH experiment for (1)H(N) and (15)N sequential correlations in ((13)C, (15)N) labelled intrinsically disordered proteins.Journal of biomolecular NMR · 2015Article
- (13)C-detected NMR experiments for automatic resonance assignment of IDPs and multiple-fixing SMFT processing.Journal of biomolecular NMR · 2015Article
- "CON-CON" assignment strategy for highly flexible intrinsically disordered proteins.Journal of biomolecular NMR · 2014Article
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2 authors.
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Abstract
NMR assignment of intrinsically disordered proteins (IDPs) by conventional HN-detected methods is hampered by the small dispersion of the amide protons chemical shifts and exchange broadening of amide proton signals. Therefore several alternative assignment strategies have been proposed in the last years. Attempting to seize that dispersion of (13)C' and (15)N chemical shifts holds even in IDPs, we recently proposed two (13)C-detected experiments to directly correlate the chemical shifts of two consecutive (13)C'-(15)N groups in proteins, i.e. without mediation of other nuclei. Main drawback of these experiments is the interruption of the connection at prolines. Here we present new (13)C-detected experiments to correlate consecutive (13)C'-(15)N groups in IDPs, hacacoNcaNCO and hacaCOncaNCO, that overcome this limitation. Moreover, the experiments provide recognition of glycine residues, thereby facilitating the assignment process.
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