ArticlePloS one2014
Differential effect of culture temperature and specific growth rate on CHO cell behavior in chemostat culture.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
- The effects of hypothermic storage on passaged chondrocyte viability and redifferentiation potential.Cytotherapy · 2026Article
- Assessing CometChip technology for DNA damage studies in non-model species: distinct UV-induced responses in turtles and mammals.BMC research notes · 2025Article
- Mammalian cell growth characterisation by a non-invasive plate reader assay.Nature communications · 2024Article
- Adaptive Laboratory Evolution to Improve Recombinant Protein Production Using Insect Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells.Adipocyte · 2023Article
- Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production.Scientific reports · 2023Article
- Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu-Jersey crossbreds.Veterinary world · 2022Article
- "Cells-to-cDNA on Chip": Phenotypic Assessment and Gene Expression Analysis from Live Cells in Nanoliter Volumes Using Droplet Microarrays.Advanced healthcare materials · 2022Article
- Article
- Asexual Blood-Stage Malaria Vaccine Candidate PfRipr5: Enhanced Production in Insect Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Dynamic Culture of Mesenchymal Stromal/Stem Cell Spheroids and Secretion of Paracrine Factors.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Improving Influenza HA-Vlps Production in Insect High Five Cells via Adaptive Laboratory Evolution.Vaccines · 2020Article
- Macrophage Stimulated by Low Ambient Temperature Hasten Tumor Growth via Glutamine Production.Biomedicines · 2020Article
- Rapid Characterization of Biomolecules' Thermal Stability in a Segmented Flow-Through Optofluidic Microsystem.Scientific reports · 2020Article
- Increased MSX level improves biological productivity and production stability in multiple recombinant GS CHO cell lines.Engineering in life sciences · 2020Article
- Impact of mammalian cell culture conditions on monoclonal antibody charge heterogeneity: an accessory monitoring tool for process development.Journal of industrial microbiology & biotechnology · 2019Article
- Automated cell segmentation in FIJI® using the DRAQ5 nuclear dye.BMC bioinformatics · 2019Article
- Adaptation of commensal proliferatingProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mild hypothermia condition in mammalian cell culture technology has been one of the main focuses of research for the development of breeding strategies to maximize productivity of these production systems. Despite the large number of studies that show positive effects of mild hypothermia on specific productivity of r-proteins, no experimental approach has addressed the indirect effect of lower temperatures on specific cell growth rate, nor how this condition possibly affects less specific productivity of r-proteins. To separately analyze the effects of mild hypothermia and specific growth rate on CHO cell metabolism and recombinant human tissue plasminogen activator productivity as a model system, high dilution rate (0.017 h(-1)) and low dilution rate (0.012 h(-1)) at two cultivation temperatures (37 and 33 °C) were evaluated using chemostat culture. The results showed a positive effect on the specific productivity of r-protein with decreasing specific growth rate at 33 °C. Differential effect was achieved by mild hypothermia on the specific productivity of r-protein, contrary to the evidence reported in batch culture. Interestingly, reduction of metabolism could not be associated with a decrease in culture temperature, but rather with a decrease in specific growth rate.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.