ArticlePloS one2014
Deep sequencing to identify the causes of viral encephalitis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Metagenomic sequencing in encephalitis diagnostics: Challenges and opportunities in clinical settings.PloS one · 2026Article
- Next-generation sequencing reveals viral aetiologies of encephalitis in Ghana: a prospective cross-sectional study.BMC infectious diseases · 2025Article
- Brain biopsy and metagenomic sequencing enhance aetiological diagnosis of encephalitis.Brain communications · 2025Article
- Prospective Cohort Study of Next-Generation Sequencing as a Diagnostic Modality for Unexplained Encephalitis in Children.Journal of the Pediatric Infectious Diseases Society · 2020Article
- Travel-associated neurological disease terminated in a postmortem diagnosed atypical HSV-1 encephalitis after high-dose steroid therapy - a case report.BMC infectious diseases · 2020Article
- Enhanced Detection of DNA Viruses in the Cerebrospinal Fluid of Encephalitis Patients Using Metagenomic Next-Generation Sequencing.Frontiers in microbiology · 2020Article
- Diagnostic Testing in Central Nervous System Infection.Seminars in neurology · 2019Review
- High-throughput sequencing for the aetiologic identification of viral encephalitis, meningoencephalitis, and meningitis. A narrative review and clinical appraisal.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2019Review
- Illumina sequencing of clinical samples for virus detection in a public health laboratory.Scientific reports · 2019Article
- Viral metagenomics revealed novel betatorquevirus species in pediatric inpatients with encephalitis/meningoencephalitis from Ghana.Scientific reports · 2019Article
- Insights From Deep Sequencing of the HBV Genome-Unique, Tiny, and Misunderstood.Gastroenterology · 2019Review
- The Feasibility of Metagenomic Next-Generation Sequencing to Identify Pathogens Causing Tuberculous Meningitis in Cerebrospinal Fluid.Frontiers in microbiology · 2019Article
- Aseptic central nervous system infections in adults: what predictor for unknown etiological diagnosis?Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2018Article
- Encephalitis diagnosis using metagenomics: application of next generation sequencing for undiagnosed cases.The Journal of infection · 2018Review
- Identification of a novel torque teno mini virus in cerebrospinal fluid from a child with encephalitis.Virologica Sinica · 2017Article
- Increased serum vascular endothelial growth factor is associated with acute viral encephalitis in Bangladeshi children.Scientific reports · 2017Article
- Enrichment of Retroviral Sequences in Brain Tissue from Patients with Severe Demyelinating Diseases.Journal of emerging diseases and virology · 2017Article
- MicroRNA-125b in peripheral blood: a potential biomarker for severity and prognosis of children with viral encephalitis.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2017Article
- Application of next generation sequencing for the detection of human viral pathogens in clinical specimens.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
- Identification of Viruses in Cases of Pediatric Acute Encephalitis and Encephalopathy Using Next-Generation Sequencing.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Deep sequencing allows for a rapid, accurate characterization of microbial DNA and RNA sequences in many types of samples. Deep sequencing (also called next generation sequencing or NGS) is being developed to assist with the diagnosis of a wide variety of infectious diseases. In this study, seven frozen brain samples from deceased subjects with recent encephalitis were investigated. RNA from each sample was extracted, randomly reverse transcribed and sequenced. The sequence analysis was performed in a blinded fashion and confirmed with pathogen-specific PCR. This analysis successfully identified measles virus sequences in two brain samples and herpes simplex virus type-1 sequences in three brain samples. No pathogen was identified in the other two brain specimens. These results were concordant with pathogen-specific PCR and partially concordant with prior neuropathological examinations, demonstrating that deep sequencing can accurately identify viral infections in frozen brain tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.