ArticlePloS one2014
Experimental infections with Mycoplasma agalactiae identify key factors involved in host-colonization.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Nucleoside binding by a surface lipoprotein governs conjugative ICE acquisition in mycoplasmas.mBio · 2026Article
- Genomic Characterization of aVeterinary sciences · 2026Article
- Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation.Frontiers in microbiology · 2023Review
- Predominant Single Stable VpmaV Expression in Strain GM139 and Major Differences withAnimals : an open access journal from MDPI · 2022Article
- Mycoplasma agalactiae ST35: a new sequence type with a minimal accessory genome primarily affecting goats.BMC veterinary research · 2022Article
- Vaccines for Mycoplasma Diseases of Small Ruminants: A Neglected Area of Research.Pathogens (Basel, Switzerland) · 2022Review
- Metal utilization in genome-reduced bacteria: Do human mycoplasmas rely on iron?Computational and structural biotechnology journal · 2021Review
- Contagious Agalactia In Sheep And Goats: Current Perspectives.Veterinary medicine (Auckland, N.Z.) · 2019Review
- Extracellular DNA: A Nutritional Trigger ofFrontiers in microbiology · 2019Article
- The Integrative Conjugative Element (ICE) ofmBio · 2018Article
- Vpma phase variation is important for survival and persistence of Mycoplasma agalactiae in the immunocompetent host.PLoS pathogens · 2017Article
- Postepizootic Persistence of Asymptomatic Mycoplasma conjunctivae Infection in Iberian Ibex.Applied and environmental microbiology · 2017Article
- Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep.Veterinary research · 2016Article
- Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection.Infection and immunity · 2015Article
- Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid.International journal of medical microbiology : IJMM · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanisms underlying pathogenic processes in mycoplasma infections are poorly understood, mainly because of limited sequence similarities with classical, bacterial virulence factors. Recently, large-scale transposon mutagenesis in the ruminant pathogen Mycoplasma agalactiae identified the NIF locus, including nifS and nifU, as essential for mycoplasma growth in cell culture, while dispensable in axenic media. To evaluate the importance of this locus in vivo, the infectivity of two knock-out mutants was tested upon experimental infection in the natural host. In this model, the parental PG2 strain was able to establish a systemic infection in lactating ewes, colonizing various body sites such as lymph nodes and the mammary gland, even when inoculated at low doses. In these PG2-infected ewes, we observed over the course of infection (i) the development of a specific antibody response and (ii) dynamic changes in expression of M. agalactiae surface variable proteins (Vpma), with multiple Vpma profiles co-existing in the same animal. In contrast and despite a sensitive model, none of the knock-out mutants were able to survive and colonize the host. The extreme avirulent phenotype of the two mutants was further supported by the absence of an IgG response in inoculated animals. The exact role of the NIF locus remains to be elucidated but these data demonstrate that it plays a key role in the infectious process of M. agalactiae and most likely of other pathogenic mycoplasma species as many carry closely related homologs.
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