Evidence map›Paper›PMID 24698983›Full record

ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2014

Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers.

Bibhuti B Das, Hua Zhang, Stanley J Opella

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Membrane protein structure from rotational diffusion.Biochimica et biophysica acta · 2015
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Bibhuti B DasDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, United States.
Hua ZhangDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, United States.
Stanley J OpellaDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, United States. Electronic address: sopella@ucsd.edu.

Funding

RESOURCE FOR SOLID STATE NMR OF PROTEINSP41EB002031 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2003 to 2018
$16.3M
Target IdentificationP01AI074805 · NIAID · FLORIDA STATE UNIVERSITY · PI CROSS, TIMOTHY A · 2009 to 2013
$8.7M
Molecular Imaging of G-Protein-Coupled Receptors for Drug DevelopmentR01EB005161 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2006 to 2014
$6.8M
Structure, Dynamics, and Functions of the Viroporins Vpu from HIV and p7 from HCVR01GM066978 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2003 to 2016
$3.5M
Structure Determination of Membrane Proteins in Phospholipid BilyaersR01GM099986 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2012 to 2015
$1.1M
NIAID NIH HHS P01 AI074805NIAID NIH HHS P01AI074805NIBIB NIH HHS P41 EB002031NIBIB NIH HHS P41EB002031NIBIB NIH HHS R01 EB005161NIBIB NIH HHS R01EB005161NIGMS NIH HHS R01GM0099986NIGMS NIH HHS R01 GM066978NIGMS NIH HHS R01GM066978NIGMS NIH HHS R01 GM099986
6 · The paper itself

Abstract

A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of heteronuclear dipolar coupling frequencies in combination with conventional chemical shift based assignment methods is demonstrated. The Dipolar Assisted Assignment Protocol (DAAP) takes advantage of the rotational alignment of the membrane proteins in liquid crystalline phospholipid bilayers. Improved resolution is obtained by combining the magnetically inequivalent heteronuclear dipolar frequencies with isotropic chemical shift frequencies. Spectra with both dipolar and chemical shift frequency axes assist with resonance assignments. DAAP can be readily extended to three- and four-dimensional experiments and to include both backbone and side chain sites in proteins.

Indexed as

AlgorithmsLipid BilayersMagnetic Resonance SpectroscopyMembrane ProteinsPhospholipidsReproducibility of ResultsSensitivity and SpecificityLipid BilayersMembrane ProteinsPhospholipidsMASMembrane proteinSolid state NMRVpu

Identifiers

PMID24698983
PMCPMC4043445

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.