Evidence map›Paper›PMID 24698832›Full record

ArticleArchives of oral biology2014

Molecular motor MYO1C, acetyltransferase KAT6B and osteogenetic transcription factor RUNX2 expression in human masseter muscle contributes to development of malocclusion.

Heather Desh, S Lauren Gray, Michael J Horton, Gwenael Raoul, Anthea M Rowlerson, Joel Ferri, Alexandre R Vieira, James J Sciote

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of oral biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Heather DeshOrthodontic Private Practice,1649 Bluebird Canyon Drive, Laguna Beach, CA, United States.
S Lauren GrayOrthodontic Department, Temple University, 3223 North Broad Street, Philadelphia, PA, United States.
Michael J HortonOrthodontic Department, Temple University, 3223 North Broad Street, Philadelphia, PA, United States.
Gwenael RaoulOral and Maxillofacial Surgery, Université Lille Nord de France, UDSL, Roger Salengro Hospital, CHU, and INSERM U 1008, Controlled Drug Delivery Systems and Biomaterials, Lille, France.
Anthea M RowlersonCentre of Human and Aerospace Physiological Sciences, King's College London, London, UK.
Joel FerriOral and Maxillofacial Surgery, Université Lille Nord de France, UDSL, Roger Salengro Hospital, CHU, and INSERM U 1008, Controlled Drug Delivery Systems and Biomaterials, Lille, France.
Alexandre R VieiraDepartment of Oral Biology, School of Dental Medicine, University of Pittsburgh, 3501 Terrace Street, Pittsburgh, PA, United States.
James J ScioteOrthodontic Department, Temple University, 3223 North Broad Street, Philadelphia, PA, United States. Electronic address: jjs6@dental.temple.edu.
Temple University · USInserm · FRKing's College London · GBUniversity of Pittsburgh · US

Funding

Musculoskeletal Heritable Influences on MalocclusionR21DE022427 · NIDCR · TEMPLE UNIV OF THE COMMONWEALTH · PI SCIOTE, JAMES J · 2012 to 2013
$415k
NIDCR NIH HHS R21 DE022427NIDCR NIH HHS R21DE022427
6 · The paper itself

Abstract

objectiveType I myosins are molecular motors necessary for glucose transport in the cytoplasm and initiation of transcription in the nucleus. Two of these, MYO1H and MYO1C, are paralogs which may be important in the development of malocclusion. The objective of this study was to investigate their gene expression in the masseter muscle of malocclusion subjects. Two functionally related proteins known to contribute to malocclusion were also investigated: KAT6B (a chromatin remodelling epigenetic enzyme which is activated by MYO1C) and RUNX2 (a transcription factor regulating osteogenesis which is activated by KAT6B).

designMasseter muscle samples and malocclusion classifications were obtained from orthognathic surgery subjects. Muscle was sectioned and immunostained to determine fibre type properties. RNA was isolated from the remaining sample to determine expression levels for the four genes by TaqMan(®) RT-PCR. Fibre type properties, gene expression quantities and malocclusion classification were compared.

resultsThere were very significant associations (P<0.0000001) between MYO1C and KAT6B expressions. There were also significant associations (P<0.005) between RUNX2 expression and masseter muscle type II fibre properties. Very few significant associations were identified between MYO1C and masseter muscle fibre type properties.

conclusionsThe relationship between MYO1C and KAT6B suggests that the two are interacting in chromatin remodelling for gene expression. This is the nuclear myosin1 (NM1) function of MYO1C. A surprising finding is the relationship between RUNX2 and type II masseter muscle fibres, since RUNX2 expression in mature muscle was previously unknown. Further investigations are necessary to elucidate the role of RUNX2 in adult masseter muscle.

Indexed as

Core Binding Factor Alpha 1 SubunitFemaleGene ExpressionHistone AcetyltransferasesHumansMaleMalocclusionMasseter MuscleMyosin Type IReal-Time Polymerase Chain ReactionYoung AdultCore Binding Factor Alpha 1 SubunitHistone AcetyltransferasesKAT6B protein, humanMYO1C protein, humanMyosin Type IRUNX2 protein, humanKAT6BMalocclusionMasseter muscleMYO1CRUNX2

Identifiers

PMID24698832
PMCPMC4049538
OpenAlexW1971962917

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.