ArticleInsect molecular biology2014
Transcriptional activation of antioxidants may compensate for selenoprotein deficiencies in Amblyomma maculatum (Acari: Ixodidae) injected with selK- or selM-dsRNA.
Article in Insect molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- De novo assembly of sialotranscriptome of Hyalomma anatolicum and insights into expression dynamics in response to Theileria annulata infection.Experimental & applied acarology · 2024Article
- Circulating oxidative stress and acute phase protein levels in horses infested with ticks.Experimental & applied acarology · 2023Article
- Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells.Biomacromolecules · 2022Article
- Redox Imbalance and Its Metabolic Consequences in Tick-Borne Diseases.Frontiers in cellular and infection microbiology · 2022Review
- Recent advances in understanding tick and rickettsiae interactions.Parasite immunology · 2021Review
- Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding.PLoS neglected tropical diseases · 2020Article
- A Peroxiredoxin From theFrontiers in cellular and infection microbiology · 2020Article
- Is selenoprotein K required for Borrelia burgdorferi infection within the tick vector Ixodes scapularis?Parasites & vectors · 2019Article
- Article
- The multiple roles of peroxiredoxins in tick blood feeding.Experimental & applied acarology · 2018Review
- Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity.Insect biochemistry and molecular biology · 2017Article
- Catalase is a determinant of the colonization and transovarial transmission of Rickettsia parkeri in the Gulf Coast tick Amblyomma maculatum.Insect molecular biology · 2017Article
- Assessment of tick antioxidant responses to exogenous oxidative stressors and insight into the role of catalase in the reproductive fitness of the Gulf Coast tick, Amblyomma maculatum.Insect molecular biology · 2016Article
- Rickettsia parkeri colonization in Amblyomma maculatum: the role of superoxide dismutases.Parasites & vectors · 2016Article
- An insight into the functional role of thioredoxin reductase, a selenoprotein, in maintaining normal native microbiota in the Gulf Coast tick (Amblyomma maculatum).Insect molecular biology · 2015Article
- Interplay between Selenium, selenoprotein genes, and oxidative stress in honey bee Apis mellifera L.Journal of insect physiologyArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The Gulf-Coast tick, Amblyomma maculatum, possesses an elaborate set of selenoproteins, which prevent the deleterious effects from oxidative stress that would otherwise occur during feeding. In the current work, we examined the role of selenoprotein K (SelK) and selenoprotein M (SelM) in feeding A. maculatum by bioinformatics, transcriptional gene expression, RNA interference and antioxidant assays. The transcriptional expression of SelK did not vary significantly in salivary glands or midguts throughout the bloodmeal. However, there was a 58-fold increase in transcript levels of SelM in tick midguts. Ticks injected with selK-dsRNA or selM-dsRNA did not reveal any observable differences in egg viability but oviposition was reduced. Surprisingly, salivary antioxidant activity was higher in selenoprotein knockouts compared with controls, which is probably the result of compensatory transcriptional expression of genes involved in combating reactive oxygen species. In fact, quantitative real-time PCR data suggest that the transcriptional expression of catalase increased in ticks injected with selM-double-stranded RNA. Additionally, the transcriptional expression of selN decreased ∼90% in both SelK/SelM knockdowns. These data indicate that SelK and SelM are salivary antioxidants but are not essential for tick survival or reproduction and are compensated by other antioxidant systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.