Evidence map›Paper›PMID 24696719›Full record

ArticleInternational journal of clinical and experimental pathology2014

VE-statin/Egfl7 expression in malignant glioma and its relevant molecular network.

Chunhai Huang, Xianrui Yuan, Yi Wan, Fei Liu, Xiaoyu Chen, Xianquan Zhan, Xuejun Li

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Chunhai HuangDepartment of Neurosurgery, First Affiliated Hospital of Jishou University Jishou 416000, Hunan, China.
Xianrui YuanDepartment of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Yi WanDepartment of Neurosurgery, First Affiliated Hospital of Jishou University Jishou 416000, Hunan, China.
Fei LiuDepartment of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Xiaoyu ChenDepartment of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Xianquan ZhanKey Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
Xuejun LiDepartment of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Central South University · CNJishou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated VE-statin/Egfl7 expression and its role and regulatory mechanism in malignant glioma progression. Forty-five paraffin-embedded glioma (grade I-II: n=24; grade III-IV: n=21) were examined. VE-statin/Egfl7 protein expression was detected via immunohistochemistry, and its correlation with pathological grade was evaluated. Three-dimensional cell culture was then performed to investigate the influence of VE-statin/Egfl7 on the angiogenesis of umbilical vein endothelial cells. Microarray detection was used to molecularly profile VE-statin/Egfl7 and relevant signaling pathways in malignant glioma (U251 cells). Data showed that VE-statin/Egfl7 protein was mainly expressed in the cytoplasm of cancer and vascular endothelial cells and was significantly related to the degree of malignancy (t=4.399, P<0.01). Additionally, VE-statin/Egfl7 expression was low in certain gray-matter neurons but undetectable in glial cells. VE-statin/Egfl7 gene silencing significantly inhibited angiogenesis in umbilical vein endothelial cells. The following microarray results were observed in VE-statin/Egfl7-silenced U251 cells: 1) EGFR family members showed the highest differential expression, accounting for 5.54% of differentially expressed genes; 2) cell survival-related signaling pathways changed significantly; and 3) the integrin ανβ3 signaling pathway was markedly altered. Thus, malignant glioma cells and glioma vascular endothelial cells highly express VE-statin/Egfl7, which is significantly correlated with the degree of malignancy. Moreover, VE-statin/Egfl7 plays an important role in glioma angiogenesis. Microarray results indicate that VE-statin/Egfl7 may regulate EGFR and integrins to influence the FAK activity of downstream factors, triggering the PI3K/Akt and Ras/MAPK cascades and subsequent malignant glioma development.

Indexed as

AdultBrain NeoplasmsCalcium-Binding ProteinsEGF Family of ProteinsEndothelial CellsEndothelial Growth FactorsFemaleGliomaHumansImmunohistochemistryMaleMiddle AgedNeovascularization, PathologicOligonucleotide Array Sequence AnalysisReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionCalcium-Binding ProteinsEGF Family of ProteinsEGFL7 protein, humanEndothelial Growth FactorsangiogenesisEpidermal growth factor-like domain 7gliomamicroarraypathological grade

Identifiers

PMID24696719
PMCPMC3971305
OpenAlexW152883170

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.