ArticleChromosoma2014
Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase.
Article in Chromosoma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex.Nature communications · 2025Article
- Kinetochore-catalyzed MCC formation: A structural perspective.IUBMB life · 2023Article
- Structure-Based Design of a Novel Class of Autotaxin Inhibitors Based on Endogenous Allosteric Modulators.Journal of medicinal chemistry · 2022Article
- Aurora B phosphorylates Bub1 to promote spindle assembly checkpoint signaling.Current biology : CB · 2022Article
- PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression.Scientific reports · 2021Article
- Chemical tools for dissecting cell division.Nature chemical biology · 2021Review
- The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.The Journal of cell biology · 2018Article
- Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling.The Journal of biological chemistry · 2018Article
- MAD1: Kinetochore Receptors and Catalytic Mechanisms.Frontiers in cell and developmental biology · 2018Review
- Optogenetic control of kinetochore function.Nature chemical biology · 2017Article
- Mps1 Regulates Kinetochore-Microtubule Attachment Stability via the Ska Complex to Ensure Error-Free Chromosome Segregation.Developmental cell · 2017Article
- A Cell Biological Perspective on Past, Present and Future Investigations of the Spindle Assembly Checkpoint.Biology · 2016Review
- How the kinetochore switches off the spindle assembly checkpoint.Cell cycle (Georgetown, Tex.) · 2016Article
- TRIP13PCH-2 promotes Mad2 localization to unattached kinetochores in the spindle checkpoint response.The Journal of cell biology · 2015Article
- Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment.Molecular biology of the cell · 2015Article
- The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.Nature cell biology · 2015Article
- The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling.Frontiers in oncology · 2015Review
- Localized light-induced protein dimerization in living cells using a photocaged dimerizer.Nature communications · 2014Article
- Signalling dynamics in the spindle checkpoint response.Nature reviews. Molecular cell biology · 2014Review
- Function and regulation of dynein in mitotic chromosome segregation.Chromosoma · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fidelity of chromosome segregation is monitored by the spindle assembly checkpoint (SAC). Key components of the SAC include MAD1, MAD2, BUB1, BUB3, BUBR1, and MPS1. These proteins accumulate on kinetochores in early prometaphase but are displaced when chromosomes attach to microtubules and/or biorient on the mitotic spindle. As a result, stable attachment of the final chromosome satisfies the SAC, permitting activation of the anaphase promoting complex/cyclosome (APC/C) and subsequent anaphase onset. SAC satisfaction is reversible, however, as addition of taxol during metaphase stops cyclin B1 degradation by the APC/C. We now show that targeting MAD1 to kinetochores during metaphase is sufficient to reestablish SAC activity after initial silencing. Using rapamycin-induced heterodimerization of FKBP-MAD1 to FRB-MIS12 and live monitoring of cyclin B1 degradation, we show that timed relocalization of MAD1 during metaphase can stop cyclin B1 degradation without affecting chromosome-spindle attachments. APC/C inhibition represented true SAC reactivation, as FKBP-MAD1 required an intact MAD2-interaction motif and MPS1 activity to accomplish this. Our data show that MAD1 kinetochore localization dictates SAC activity and imply that SAC regulatory mechanisms downstream of MAD1 remain functional in metaphase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.