ArticleThe EMBO journal1989
Enhancer elements directing cell-type-specific expression of cytokeratin genes and changes of the epithelial cytoskeleton by transfections of hybrid cytokeratin genes.
Article in The EMBO journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 98 citations in OpenAlex.
- Skin Carcinogenesis Studies Using Mouse Models with Altered Polyamines.Cancer growth and metastasis · 2015Review
- Skin hyperproliferation and susceptibility to chemical carcinogenesis in transgenic mice expressing E6 and E7 of human papillomavirus type 38.Journal of virology · 2005Article
- A 5'-upstream region of a bovine keratin 6 gene confers tissue-specific expression and hyperproliferation-related induction in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Construction and properties of pseudorabies virus recombinants with altered control of immediate-early gene expression.Journal of virology · 1995Article
- Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.The Journal of cell biology · 1995Article
- Targeted expression of the E6 and E7 oncogenes of human papillomavirus type 16 in the epidermis of transgenic mice elicits generalized epidermal hyperplasia involving autocrine factors.Molecular and cellular biology · 1994Article
- The human 230-kD bullous pemphigoid antigen gene (BPAG1). Exon-intron organization and identification of regulatory tissue specific elements in the promoter region.The Journal of clinical investigation · 1993Article
- Probing keratinocyte and differentiation specificity of the human K5 promoter in vitro and in transgenic mice.Molecular and cellular biology · 1993Article
- Ectopic synthesis of epidermal cytokeratins in pancreatic islet cells of transgenic mice interferes with cytoskeletal order and insulin production.The Journal of cell biology · 1993Article
- Regulation of the mouse desmin gene: transactivated by MyoD, myogenin, MRF4 and Myf5.Nucleic acids research · 1993Article
- Profilaggrin is a major epidermal calcium-binding protein.Molecular and cellular biology · 1993Article
- Expression of the carcinoma-associated keratin K6 and the role of AP-1 proto-oncoproteins.Gene expression · 1993Article
- Transcription factor AP2 and its role in epidermal-specific gene expression.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Positive selection of candidate tumor-suppressor genes by subtractive hybridization.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Epidermal differentiation: the bare essentials.The Journal of cell biology · 1990Review
- Differential extraction of keratin subunits and filaments from normal human epidermis.The Journal of cell biology · 1990Article
- Epithelial-specific keratin gene expression: identification of a 300 base-pair controlling segment.Nucleic acids research · 1990Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytokeratins, which form the intermediate filaments (IFs) characteristic of epithelial cells, are encoded by a large family of genes whose members are differentially expressed in patterns different in the various kinds of epithelia. To identify possible cis-regulatory DNA elements involved in the cell-type-specific expression of these genes, we examined, in transfection assays, 5' upstream sequence intercepts of a certain cytokeratin gene, i.e. that for bovine cytokeratin IV* (CKIV*), in combination with the coding portions of either the chloramphenicol acetyltransferase (CAT) gene or other cytokeratin genes. A 5' upstream region located between the cap-site and nucleotide -605 was found to enhance the specific expression of these reporter genes in bovine mammary gland-derived BMGE + H cells, which express the endogenous gene, but not in bovine kidney epithelium-derived MDBK cells which synthesize cytokeratins other than IV*. This epithelium-type-specific expression was also observed in heterologous combinations, e.g. in murine keratinocytes, but not in other murine cell lines such as 3T3 fibroblasts. When a fragment located between -180 and -605 was coupled to the HSV-TK promoter it stimulated the expression of the reporter gene in a cell-type-specific manner. The enhancer character of this 425 nucleotide long region is also demonstrated. Moreover, the CKIV* promoter/enhancer complex was able to direct the expression of epidermal cytokeratins characteristic for suprabasal differentiation, i.e. bovine cytokeratins Ia and VIb, in cells that normally do not express these genes. We show that the newly synthesized cytokeratins integrate into the pre-existing cytokeratin IF system of the transfected cells and that the forced expression of one of these cytokeratins does not induce the endogenous gene encoding its normal pair partner.
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