ArticleJournal of lipid research2014
Static and turnover kinetic measurement of protein biomarkers involved in triglyceride metabolism including apoB48 and apoA5 by LC/MS/MS.
Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A high-throughput mass spectrometry-based assay for large-scale profiling of circulating human apolipoproteins.Journal of lipid research · 2020Article
- Investigation of human apoB48 metabolism using a new, integrated non-steady-state model of apoB48 and apoB100 kinetics.Journal of internal medicine · 2019 · on this mapArticle
- Effects of APOC3 Heterozygous Deficiency on Plasma Lipid and Lipoprotein Metabolism.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Kinetics of plasma apolipoprotein E isoforms by LC-MS/MS: a pilot study.Journal of lipid research · 2018Article
- Effects of CETP inhibition with anacetrapib on metabolism of VLDL-TG and plasma apolipoproteins C-II, C-III, and E.Journal of lipid research · 2017Article
- Multiplexed peptide analysis for kinetic measurements of major human apolipoproteins by LC/MS/MS.Journal of lipid research · 2016Article
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Authors and funding
19 authors.
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Abstract
LC/MS quantification of multiple plasma proteins that differ by several orders of magnitude in concentration from a single sample is challenging. We present a strategy that allows the simultaneous determination of the concentration and turnover kinetics of higher and lower abundant proteins from a single digestion mixture. Our attention was directed at a cluster of proteins that interact to affect the absorption and interorgan lipid trafficking. We demonstrate that apos involved in TG metabolism such as apoC2, C3, E, and A4 (micromolar concentration), and apoB48 and apoA5 (single-digit nanomolar concentration) can be quantified from a single digestion mixture. A high degree of correlation between LC/MS and immunobased measurements for apoC2, C3, E, and B48 was observed. Moreover, apoA5 fractional synthesis rate was measured in humans for the first time. Finally, the method can be directly applied to studies involving nonhuman primates because peptide sequences used in the method are conserved between humans and nonhuman primates.
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